Although the association between maternal periconceptional diet and adult offspring health is well characterised, our understanding of the impact of paternal nutrition at the time of conception on offspring phenotype remains poorly defined. Therefore, we determined the effect of a paternal preconception
low protein diet (LPD) on adult offspring cardiovascular and metabolic health in mice. Male C57BL/6 mice were fed either normal
protein diet (
NPD; 18%
casein) or LPD (9%
casein) for 7 wk before mating. At birth, a reduced male-to-female ratio (P = 0.03) and increased male offspring weight (P = 0.009) were observed in litters from LPD compared with
NPD stud males with no differences in mean litter size. LPD offspring were heavier than
NPD offspring at 2 and 3 wk of age (P < 0.02). However, no subsequent differences in
body weight were observed. Adult male offspring derived from LPD studs developed relative
hypotension (decreased by 9.2 mmHg) and elevated heart rate (P < 0.05), whereas both male and female offspring displayed vascular dysfunction and
impaired glucose tolerance relative to
NPD offspring. At cull (24 wk), LPD males had elevated adiposity (P = 0.04), reduced heart-to-
body weight ratio (P = 0.04), and elevated circulating TNF-α levels (P = 0.015) compared with
NPD males. Transcript expression in offspring heart and liver tissue was reduced for genes involved in calcium signaling (Adcy, Plcb, Prkcb) and metabolism (Fto) in LPD offspring (P < 0.03). These novel data reveal the impact of suboptimal paternal nutrition on adult offspring cardiovascular and metabolic homeostasis, and provide some insight into the underlying regulatory mechanisms.