Acetyl-11-keto-beta-BA (AKBA), a component of the gum resin of Boswellia serrata, has been recognized as a promising agent for the prevention of intestinal
tumorigenesis.
Aspirin, a non-steroidal anti-inflammatory
drug (
NSAID), has also been considered to have the activity against intestinal
tumorigenesis. However, the prevention of
colonic cancer is insufficient and no definitive recommendation has been made for clinic use. Herein, we compared the efficacy of AKBA with that of
aspirin in an
adenomatous polyposis coli intestinal
neoplasia consecutive weeks. Mice were sacrificed by anesthetizing. The whole intestine was removed from each mouse. The number, size and histopathology of intestinal
adenomatous polyps were examined under microscopy. The
adenomatous polyps were removed for further analysis by the assays of western blotting and immunohistochemical staining. AKBA significantly prevented the formation of intestinal
adenomatous polyps without toxicity to mice. Statistical analysis indicated that AKBA's activity both in the prevention of small intestinal and
colonic polyps was more potently than
aspirin. Histopathologic examination revealed that AKBA's effect, that is the reduction of
polyp size and degree of dysplasia, was more prominent in larger sized
polyps, especially those originating in colon. These effects of AKBA were associated with its role in the induction of apoptosis in
carcinomas. The assays of western blotting and immunohistochemistry staining indicated that the efficacy of AKBA might arise from its activity in the modulation of the Wnt/β-
catenin pathway and NF-κB/COX-2 pathway in
adenomatous polyps. Conclusion, AKBA by oral application prevented intestinal
tumorigenesis more potential than
aspirin.