Evaluation of Institut Georges Lopez-1 preservation solution in pig pancreas transplantation: a pilot study.

Abstract

BACKGROUND:

Institut Georges Lopez-1 preservation solution (IGL-1) is an emerging extracellular-type electrolyte solution, low in viscosity, containing polyethylene glycol 35 as a colloid. Although IGL-1 has shown beneficial outcomes in kidney and liver preservation, this pilot study is the first to evaluate the efficacy of IGL-1 in pancreas transplantation (PT) compared with the University of Wisconsin solution (UW).

METHODS:

Sixteen Landrace pigs underwent allogeneic PT with 16 hr of cold ischemia. Grafts were preserved with IGL-1 (n=8) or UW (n=8). No immunosuppression was administered. We analyzed graft function, the acute-phase response, and oxidative stress in the pancreatic graft monitoring membrane fluidity and lipid peroxidation.

RESULTS:

All eight grafts with IGL-1, but only six with UW, were functioning. Graft failures with UW resulted from graft thrombosis. There were no differences between the two solutions in the number of normoglycemic days (IGL-1: 11.5 ± 6.2 versus UW: 8.5 ± 4.4 days, P=0.1357), nor in lipid peroxidation during 16-hr cold ischemia (P=0.672), or reperfusion (P=0.185), but IGL-1 prevented changes in membrane fluidity after reperfusion when compared with UW (P=0.026).

CONCLUSION:

IGL-1 offered the same degree of safety and effectiveness as UW in our model of pig PT with 16 hr of cold ischemia.

• Authors: Francisco A García-Gil, Lorena Fuentes-Broto, Carlos D Albendea, María Trinidad Serrano, Joan Roselló-Catafau, Fermín Lampreave, Laura López-Pingarrón, Jorge Escartín, Joaquín Soria, Joaquín J Garcia, Laureano Fernández-Cruz
• Journal: Transplantation (Transplantation) Vol. 97 Issue 9 Pg. 901-7 (May 15 2014) ISSN: 1534-6080 [Electronic] United States
• PMID: 24646772 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Colloids
• Electrolytes
• IGL-1 solution
• Insulin
• Organ Preservation Solutions
• University of Wisconsin-lactobionate solution
• Polyethylene Glycols
• Allopurinol
• Glutathione
• Adenosine
• Raffinose

Topics

• Adenosine (chemistry)
• Allopurinol (chemistry)
• Animals
• Colloids (chemistry)
• Electrolytes
• Female
• Glutathione (chemistry)
• Immunosuppression Therapy
• Insulin (chemistry)
• Ischemia
• Kidney (pathology)
• Lipid Peroxidation
• Liver (pathology)
• Organ Preservation (methods)
• Organ Preservation Solutions (chemistry)
• Oxidative Stress
• Pancreas (pathology)
• Pancreas Transplantation (methods)
• Pilot Projects
• Polyethylene Glycols (chemistry)
• Raffinose (chemistry)
• Swine
• Time Factors
• Viscosity