Abstract |
The main pathological characteristic of glomerulonephritis is diffuse mesangial cell proliferation. MiR-34a is associated with the proliferation of various organs and cancer cells. However, the role of miR-34a in renal proliferation diseases is not clear. Therefore, this study aimed to elucidate the mechanism of miR-34a in the regulation of renal mesangial cell proliferation. The miR-34a expression level at different time points in an anti-Thy1 mesangial proliferative nephritis rat model was determined by qRT-PCR. The cell proliferation rate and cell cycle changes were measured in the in vitro cultured rat mesangial cells (RMCs). Our results suggested that miR-34a expression was negatively correlated with the degree of cell proliferation in the anti-Thy1 nephritis model. MiR-34a could extend the G0/G1 phase and block cell proliferation in RMCs. Dual- luciferase assay results showed that there were binding sites of miR-34a at 3'-UTR of platelet-derived growth factor receptor-β (PDGFR-β). MiR-34a can inhibit PDGFR-β protein expression at a post-transcriptional level, suppress Ras/MAPK signaling pathways, and down-regulate expression of cell cycle proteins at the G0/G1 phase, such as cyclin D1, CDK4/CDK6. In addition, miR-34a may also inhibit RMC proliferation by directly targeting cyclin E and CDK2. MiR-34a inhibits exogenous stimuli-induced proliferation of mesangial cells. Expression levels of phospho-PDGFR-β and phospho-MEK1 (an important downstream molecule in PDGFR-β-induced signaling pathway) were significantly increased in the anti-Thy-1 nephritis rat model. These results suggest that miR-34a may regulate RMC proliferation by directly inhibiting expressions of PDGFR-β, MEK1, and cell cycle proteins, cyclin E and CDK2.
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Authors | Dapeng Chen, Ying Li, Yan Mei, Wenjia Geng, Jurong Yang, Quan Hong, Zhe Feng, Guangyan Cai, Hanyu Zhu, Suozhu Shi, Xue-Yuan Bai, Xiangmei Chen |
Journal | Cellular and molecular life sciences : CMLS
(Cell Mol Life Sci)
Vol. 71
Issue 20
Pg. 4027-42
(Oct 2014)
ISSN: 1420-9071 [Electronic] Switzerland |
PMID | 24638095
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3' Untranslated Regions
- Cell Cycle Proteins
- Isoantibodies
- MIRN34 microRNA, rat
- MicroRNAs
- RNA, Small Interfering
- anti-Thy antibody
- Receptor, Platelet-Derived Growth Factor beta
- Mitogen-Activated Protein Kinase Kinases
- ras Proteins
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Topics |
- 3' Untranslated Regions
- Animals
- Cell Cycle Proteins
(genetics, metabolism)
- Cell Line
- Cell Proliferation
- G1 Phase
- Glomerulonephritis
(metabolism, pathology)
- Isoantibodies
(immunology)
- Mesangial Cells
(cytology, metabolism)
- MicroRNAs
(metabolism)
- Mitogen-Activated Protein Kinase Kinases
(metabolism)
- RNA Interference
- RNA, Small Interfering
(metabolism)
- Rats
- Rats, Wistar
- Receptor, Platelet-Derived Growth Factor beta
(antagonists & inhibitors, genetics, metabolism)
- Signal Transduction
- ras Proteins
(metabolism)
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