Therapeutic potential of the antimicrobial peptide OH-CATH30 for antibiotic-resistant Pseudomonas aeruginosa keratitis.

Abstract

The therapeutic potential of antimicrobial peptides (AMPs) has been evaluated in many infectious diseases. However, the topical application of AMPs for ocular bacterial infection has not been well investigated. The AMP OH-CATH30, which was identified in the king cobra, exhibits potent antimicrobial activity. In this study, we investigated the therapeutic potential of OH-CATH30 for Pseudomonas aeruginosa keratitis. Ten isolates of P. aeruginosa from individuals with keratitis were susceptible to OH-CATH30 but not to cefoperazone, ciprofloxacin, gentamicin, and levofloxacin. The microdilution checkerboard assay showed that OH-CATH30 exhibited synergistic activity with ciprofloxacin and levofloxacin against antibiotic-resistant P. aeruginosa. Meanwhile, P. aeruginosa did not develop resistance to OH-CATH30, even after exposure at 0.5× the MIC for up to 25 subcultures. Furthermore, treatment with OH-CATH30, alone or in combination with levofloxacin, significantly improved the clinical outcomes of rabbit keratitis induced by antibiotic-resistant P. aeruginosa. Taken together, our data indicate that the topical application of OH-CATH30 is efficacious against drug-resistant P. aeruginosa keratitis. In addition, our study highlights the potential application of AMPs in treating ocular bacterial infections.

• Authors: Sheng-An Li, Jie Liu, Yang Xiang, Yan-Jie Wang, Wen-Hui Lee, Yun Zhang
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 58 Issue 6 Pg. 3144-50 (Jun 2014) ISSN: 1098-6596 [Electronic] United States
• PMID: 24637683 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Chemical References

• Anti-Bacterial Agents
• Anti-Infective Agents
• Antimicrobial Cationic Peptides
• Gentamicins
• Ciprofloxacin
• Levofloxacin

Topics

• Administration, Topical
• Animals
• Anti-Bacterial Agents (pharmacology)
• Anti-Infective Agents (pharmacology)
• Antimicrobial Cationic Peptides (pharmacology)
• Ciprofloxacin (pharmacology)
• Disease Models, Animal
• Drug Resistance, Bacterial
• Drug Synergism
• Eye Infections, Bacterial (drug therapy, microbiology)
• Gentamicins (pharmacology)
• Humans
• Keratitis (drug therapy, microbiology)
• Levofloxacin (pharmacology)
• Microbial Sensitivity Tests
• Pseudomonas Infections (drug therapy, microbiology)
• Pseudomonas aeruginosa (drug effects)
• Rabbits