Ischaemia-related cell damage in extracorporeal preserved tissue - new findings with a novel perfusion model.

Abstract

Tissue undergoing free transfer in transplant or reconstructive surgery always is at high risk of ischaemia-related cell damage. This study aims at assessing different procedures using an extracorporeal perfusion and oxygenation system to investigate the expression of hypoxia inducible factor (HIF)-1-α as marker for hypoxia and of the pro-apoptotic protein Caspase-3 in skeletal muscle to elucidate potential improvements in tissue conservation. Twenty-four porcine rectus abdominis muscles were assigned to five different groups and examined after they had been extracorporeally preserved for 60 min. time. Group I was left untreated (control), group II was perfused with a cardioplegic solution, group III was flushed with 10 ml of a cardioplegic solution and then left untreated. Group IV and V were perfused and oxygenated with either an isotone crystalloid solution or a cardioplegic solution. Among others, immunohistochemistry (Caspase-3 and HIF-1-α) of muscle samples was performed. Furthermore, oxygen partial pressure in the perfusate at the arterial and venous branch was measured. Expression of Caspase-3 after 60 min. was reduced in all groups compared to the control group. Furthermore, all groups (except group III) expressed less HIF-1-α than the control group. Oxygenation leads to higher oxygen levels at the venous branch compared to groups without oxygenation. Using an extracorporeal perfusion and oxygenation system cell damage could be reduced as indicated by stabilized expressions of Caspase-3 and HIF-1-α for 60 min. of tissue preservation. Complete depletion of oxygen at the venous branch can be prevented by oxygenation of the perfusate with ambient air.

• Authors: Christian D Taeger, Wibke Müller-Seubert, Raymund E Horch, Konstantin Präbst, Frank Münch, Carol I Geppert, Torsten Birkholz, Adrian Dragu
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 18 Issue 5 Pg. 885-94 (May 2014) ISSN: 1582-4934 [Electronic] England
• PMID: 24636195 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• Hypoxia-Inducible Factor 1, alpha Subunit
• Caspase 3
• Oxygen

Topics

• Animals
• Arteries (metabolism)
• Caspase 3 (metabolism)
• Hydrogen-Ion Concentration
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Immunohistochemistry
• Ischemia (pathology)
• Male
• Models, Animal
• Oxygen (metabolism)
• Partial Pressure
• Perfusion
• Preservation, Biological
• Staining and Labeling
• Sus scrofa
• Veins (metabolism)