Simultaneous detection of IgG antibodies associated with viral hemorrhagic fever by a multiplexed Luminex-based immunoassay.

Abstract

Viral hemorrhagic fevers (VHFs) are worldwide diseases caused by several kinds of viruses. With the emergence of new viruses, advanced diagnostic methods are urgently needed for identification of VHFs. Based on Luminex xMAP technology, a rapid, sensitive, multi-pathogen and high-throughput method which could simultaneously detect hemorrhagic fever viruses (HFVs) specific IgG antibodies was developed. Recombinant antigens of nine HFVs including Hantaan virus (HTNV), Seoul virus (SEOV), Puumala virus (PUUV), Andes virus (ANDV), Sin Nombre virus (SNV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) and dengue virus (DENV) were produced and purified from a prokaryotic expression system and the influence of the coupling amount was investigated. Cross-reactions among antigens and their rabbit immune sera were evaluated. Serum samples collected from 51 laboratory confirmed hemorrhagic fever with renal syndrome (HFRS) patients, 43 confirmed SFTS patients and 88 healthy donors were analyzed. Results showed that recombinant nucleocapsid protein of the five viruses belonging to the genus Hantavirus, had serological cross-reactivity with their corresponding rabbit immune sera, but not apparent with immune sera of other four viruses. Evaluation of this new method with clinical serum samples showed 98.04% diagnostic sensitivity for HFRS, 90.70% for SFTS detection and the specificity was ranging from 66.67% to 100.00%. The multiplexed Luminex-based immunoassay has firstly been established in our study, which provides a potentially reliable diagnostic tool for IgG antibody detection of VHFs.

• Authors: Wei Wu, Shuo Zhang, Jing Qu, Quanfu Zhang, Chuan Li, Jiandong Li, Cong Jin, Mifang Liang, Dexin Li
• Journal: Virus research (Virus Res) Vol. 187 Pg. 84-90 (Jul 17 2014) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 24631566 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Viral
• Immunoglobulin G

Topics

• Animals
• Antibodies, Viral (blood)
• Cross Reactions
• Dengue Virus (immunology, isolation & purification)
• Orthohantavirus (classification, immunology, isolation & purification)
• Hemorrhagic Fever Virus, Crimean-Congo (immunology, isolation & purification)
• Hemorrhagic Fevers, Viral (blood, diagnosis, immunology, virology)
• Humans
• Immunoassay
• Immunoglobulin G (blood)
• Phlebovirus (immunology, isolation & purification)
• Rabbits
• Rift Valley fever virus (immunology, isolation & purification)
• Sensitivity and Specificity