Abstract |
We previously demonstrated that mRNA for the pro-inflammatory cytokine interleukin 20 (IL-20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin (LS). Here, we describe the distribution of IL-20 protein and the identity of the IL-20-positive cells in LS. We found that the main part of IL-20 immunoreactivity is present in mononuclear cells of the dermal papillae, and that the IL-20-positive cells located in the papillae were langerin+, CD1a+, CD4+ and CD303+. These cells might be immature dendritic cell. In situ hybridization for IL-20 mRNA on non-LS, ex vivo stimulated with IL-1β revealed a colocalization between IL-20 mRNA and the keratinocyte marker CK14. No IL-20 mRNA was detected in the dermal mononuclear cells. Our results suggest that IL-20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells. Our study supports that IL-20 is involved in the pathogenesis of psoriasis.
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Authors | Rikke Bech, Kristian Otkjaer, Svend Birkelund, Thomas Vorup-Jensen, Ralf Agger, Claus Johansen, Lars Iversen, Knud Kragballe, John Rømer |
Journal | Experimental dermatology
(Exp Dermatol)
Vol. 23
Issue 5
Pg. 349-52
(May 2014)
ISSN: 1600-0625 [Electronic] Denmark |
PMID | 24628979
(Publication Type: Letter, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Antigens, CD
- Antigens, CD1
- CD1a antigen
- CD207 protein, human
- CD4 Antigens
- CLEC4C protein, human
- Interleukin-1beta
- Interleukins
- Keratin-14
- Lectins, C-Type
- Mannose-Binding Lectins
- Membrane Glycoproteins
- Receptors, Immunologic
- interleukin 20
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Topics |
- Antigens, CD
(metabolism)
- Antigens, CD1
(metabolism)
- CD4 Antigens
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Epidermis
(metabolism)
- HEK293 Cells
- Humans
- Interleukin-1beta
(metabolism)
- Interleukins
(metabolism)
- Keratin-14
(metabolism)
- Keratinocytes
(metabolism)
- Lectins, C-Type
(metabolism)
- Leukocytes, Mononuclear
(metabolism)
- Mannose-Binding Lectins
(metabolism)
- Membrane Glycoproteins
(metabolism)
- Psoriasis
(metabolism)
- Receptors, Immunologic
(metabolism)
- Skin
(metabolism)
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