Presence of a charybdotoxin sensitive Ca2+-activated K+ channel in rat glioma C6 cells.

Abstract

A study was made of the 86Rb+ influx and efflux through Ca2+-activated K+ channels of intact rat glioma C6 cells after addition of a Ca2+ ionophore to the incubation medium. Half-maximal activation of the channels was obtained at a cytoplasmic Ca2+ concentration of approximately 400 nM. The 86Rb+ ion flux through the Ca2+-activated K+ channels was insensitive to apamin, but was inhibited by low concentrations of charybdotoxin (IC50 = 1.6 nM). This is the first evidence for the presence of charybdotoxin-sensitive Ca2+-activated K+ channels in glial cells.

• Authors: P W Tas, H G Kress, K Koschel
• Journal: Neuroscience letters (Neurosci Lett) Vol. 94 Issue 3 Pg. 279-84 (Dec 05 1988) ISSN: 0304-3940 [Print] Ireland
• PMID: 2462699 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ethers
• Potassium Channels
• Rubidium Radioisotopes
• Scorpion Venoms
• Charybdotoxin
• Calcimycin
• Ionomycin
• Rubidium
• Calcium

Topics

• Animals
• Calcimycin (pharmacology)
• Calcium (pharmacology)
• Charybdotoxin
• Ethers (pharmacology)
• Glioma (metabolism, pathology)
• Ionomycin
• Potassium Channels (metabolism)
• Rubidium (antagonists & inhibitors, metabolism)
• Rubidium Radioisotopes
• Scorpion Venoms (pharmacology)
• Tumor Cells, Cultured