Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Thirty naturally infected mongrel dogs displaying clinical signs of visceral leishmaniasis were randomly divided in three groups: one group received three doses of rLdccys1 in combination with the adjuvant Propionibacterium acnes at one month interval between each dose; a second group received three doses of P. acnes alone; a third group received saline. The main findings were: 1) dogs that received rLdccys1 with P. acnes did not display increase of the following clinical signs: weight loss, alopecia, onychogryphosis, cachexia, anorexia, apathy, skin lesions, hyperkeratosis, ocular secretion, and enlarged lymph nodes; they also exhibited a significant reduction in the spleen parasite load in comparison to the control dogs; 2) rLdccys1-treated dogs exhibited a significant delayed type cutaneous hypersensitivity elicited by the recombinant antigen, as well as high IgG2 serum titers and low IgG1 serum titers; sera from rLdccys1-treated dogs also contained high IFN-γ and low IL-10 concentrations; 3) control dogs exhibited all of the clinical signs of visceral leishmaniasis and had low serum IgG2 and IFN-γ levels and high concentrations of IgG1 and IL-10; 4) all of the dogs treated with rLdccys1 were alive 12 months after treatment, whereas dogs which received either saline or P. acnes alone died within 3 to 7 months. CONCLUSIONS/SIGNIFICANCE:
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Authors | Josie Haydée Lima Ferreira, Lucilene Dos Santos Silva, Ieda Maria Longo-Maugéri, Simone Katz, Clara Lúcia Barbiéri |
Journal | PLoS neglected tropical diseases
(PLoS Negl Trop Dis)
Vol. 8
Issue 3
Pg. e2729
(Mar 2014)
ISSN: 1935-2735 [Electronic] United States |
PMID | 24625516
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Protozoan
- Cytokines
- Protozoan Proteins
- Recombinant Proteins
- Cysteine Proteases
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Topics |
- Animals
- Antibodies, Protozoan
(blood)
- Brazil
- Cysteine Proteases
(genetics, therapeutic use)
- Cytokines
(blood)
- Dog Diseases
(pathology, therapy)
- Dogs
- Immunotherapy
(methods)
- Leishmania infantum
(enzymology)
- Leishmaniasis, Visceral
(pathology, therapy, veterinary)
- Male
- Mice
- Protozoan Proteins
(genetics, therapeutic use)
- Recombinant Proteins
(genetics, therapeutic use)
- Survival Analysis
- Treatment Outcome
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