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Simultaneous NF-κB inhibition and E-cadherin upregulation mediate mutually synergistic anticancer activity of celastrol and SAHA in vitro and in vivo.

Abstract
Suberoylanilide hydroxamic acid (SAHA) is a promising histone deacetylase (HDAC) inhibitor approved by the US Food and Drug Administration (FDA) and whose clinical application for solid tumours is partially limited by decreased susceptibility in cancer cells due to nuclear factor (NF)-κB activation. As an NF-κB inhibitor, celastrol exhibits potent anticancer effects but has failed to enter clinical trials due to its toxicity. In this report, we demonstrated that the combination of celastrol and SAHA exerted substantial synergistic efficacy against human cancer cells in vitro and in vivo accompanied by enhanced caspase-mediated apoptosis. This drug combination inhibited the activation of NF-κB caused by SAHA monotherapy and consequently led to increased apoptosis in cancer cells. Interestingly, E-cadherin was dramatically downregulated in celastrol-resistant cancer cells, and E-cadherin expression was closely related to decreased sensitivity to celastrol. However, our combination treatment significantly augmented the expression of E-cadherin, suggesting that mutual mechanisms contributed to the synergistic anticancer activity. Furthermore, the enhanced anticancer efficacy of celastrol combined with SAHA was validated in a human lung cancer 95-D xenograft model without increased toxicity. Taken together, our data demonstrated the synergistic anticancer effects of celastrol and SAHA due to their reciprocal sensitisation, which was simultaneously regulated by NF-κB and E-cadherin; thus, the combination of celastrol and SAHA was superior to other combination regimens that rely on a single mechanism. Our findings not only open new opportunities for the clinical development of SAHA but should also motivate the clinical investigation of celastrol, which has been hampered by its toxicity.
AuthorsLin Zheng, Yingying Fu, Linhan Zhuang, Renhua Gai, Jian Ma, Jianshu Lou, Hong Zhu, Qiaojun He, Bo Yang
JournalInternational journal of cancer (Int J Cancer) Vol. 135 Issue 7 Pg. 1721-32 (Oct 01 2014) ISSN: 1097-0215 [Electronic] United States
PMID24615207 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 UICC.
Chemical References
  • Cadherins
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • NF-kappa B
  • Pentacyclic Triterpenes
  • RNA, Messenger
  • Triterpenes
  • Vorinostat
  • celastrol
Topics
  • Animals
  • Apoptosis (drug effects)
  • Blotting, Western
  • Cadherins (genetics, metabolism)
  • Cell Proliferation (drug effects)
  • Drug Synergism
  • Female
  • Flow Cytometry
  • Gene Expression Regulation, Neoplastic
  • Histone Deacetylase Inhibitors (pharmacology)
  • Humans
  • Hydroxamic Acids (pharmacology)
  • Immunoenzyme Techniques
  • In Vitro Techniques
  • Lung Neoplasms (drug therapy, metabolism, pathology)
  • Mice
  • Mice, Nude
  • NF-kappa B (antagonists & inhibitors, genetics, metabolism)
  • Pentacyclic Triterpenes
  • RNA, Messenger (genetics)
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tripterygium (chemistry)
  • Triterpenes (pharmacology)
  • Tumor Cells, Cultured
  • Vorinostat

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