Abstract | AIM: MATERIALS AND METHODS: The content of separate antigens ( pertussis toxin, filamentous hemagglutinin and agglutinogens 1, 2, 3) in samples of acellular pertussis component of ADTaP- vaccine and completeness of sorption of pertussis component of ADTaP- vaccine were evaluated by using enzyme immunoassay. Completeness of sorption of diphtheria and tetanus components were determined in flocculation reaction and antitoxin-binding reactions, respectively. Protective activity ofADTaP- vaccine was studied in model ofmeningoencephalitis development in mice infected with Bordetella pertussis (strain 18323) neurotropic virulent culture, protective activity oftetanus component - by survival of mice after administration of tetanus toxin, protective activity of diphtheria component - by survival of guinea pigs after administration of diphtheria toxin. Safety of preparations was evaluated in tests of acute and chronic toxicity with carrying out pathomorphologic studies including immature animals. RESULTS: All the studied experimental series ofADTaP- vaccine were standard by content of separate antigens of pertussis microbe. All the ADTaP- vaccine components were completely sorbed on aluminium hydroxide gel. By protective activity ADTaP preparations satisfied the WHO requirements. The preparations were non-toxic in acute and chronic toxicity and did not induce pathomorphologic changes including immature animals. CONCLUSION: Experimental samples of ADTaP- vaccine by specific activity and safety satisfied WHO requirements.
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Authors | E M Zaĭtsev, M V Britsina, I G Bazhanova, N U Mertsalova, M N Ozeretskovskaia, E V Ermolova, N G Plekhanova, N A Mikhaĭlova, V A Kolyshkin, V V Zverev |
Journal | Zhurnal mikrobiologii, epidemiologii i immunobiologii
(Zh Mikrobiol Epidemiol Immunobiol)
2013 Nov-Dec
Issue 6
Pg. 44-9
ISSN: 0372-9311 [Print] Russia (Federation) |
PMID | 24605674
(Publication Type: Journal Article)
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Chemical References |
- Adjuvants, Immunologic
- Antigens, Bacterial
- Diphtheria Toxin
- Diphtheria-Tetanus-acellular Pertussis Vaccines
- Tetanus Toxin
- Aluminum Hydroxide
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Topics |
- Adjuvants, Immunologic
(adverse effects, pharmacology)
- Aluminum Hydroxide
(adverse effects, pharmacology)
- Animals
- Antigens, Bacterial
(adverse effects, immunology, pharmacology)
- Bordetella pertussis
- Diphtheria Toxin
(immunology, toxicity)
- Diphtheria-Tetanus-acellular Pertussis Vaccines
(adverse effects, immunology, pharmacology)
- Drug Evaluation, Preclinical
- Female
- Guinea Pigs
- Humans
- Male
- Meningoencephalitis
(immunology, prevention & control)
- Mice
- Tetanus Toxin
(immunology, toxicity)
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