HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Preclinical studies of an adsorbed diphtheria-tetanus-pertussis vaccine (ADTP-vaccine) with acellular pertussis component].

AbstractAIM:
Evaluate standardness of antigenic composition of pertussis component, completeness of sorption of pertussis, diphtheria and tetanus components, specific activity and safety of experimental series ofADTP-vaccine with acellular pertussis component (ADTaP-vaccine).
MATERIALS AND METHODS:
The content of separate antigens (pertussis toxin, filamentous hemagglutinin and agglutinogens 1, 2, 3) in samples of acellular pertussis component of ADTaP-vaccine and completeness of sorption of pertussis component of ADTaP-vaccine were evaluated by using enzyme immunoassay. Completeness of sorption of diphtheria and tetanus components were determined in flocculation reaction and antitoxin-binding reactions, respectively. Protective activity ofADTaP-vaccine was studied in model ofmeningoencephalitis development in mice infected with Bordetella pertussis (strain 18323) neurotropic virulent culture, protective activity oftetanus component - by survival of mice after administration of tetanus toxin, protective activity of diphtheria component - by survival of guinea pigs after administration of diphtheria toxin. Safety of preparations was evaluated in tests of acute and chronic toxicity with carrying out pathomorphologic studies including immature animals.
RESULTS:
All the studied experimental series ofADTaP-vaccine were standard by content of separate antigens of pertussis microbe. All the ADTaP-vaccine components were completely sorbed on aluminium hydroxide gel. By protective activity ADTaP preparations satisfied the WHO requirements. The preparations were non-toxic in acute and chronic toxicity and did not induce pathomorphologic changes including immature animals.
CONCLUSION:
Experimental samples of ADTaP-vaccine by specific activity and safety satisfied WHO requirements.
AuthorsE M Zaĭtsev, M V Britsina, I G Bazhanova, N U Mertsalova, M N Ozeretskovskaia, E V Ermolova, N G Plekhanova, N A Mikhaĭlova, V A Kolyshkin, V V Zverev
JournalZhurnal mikrobiologii, epidemiologii i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) 2013 Nov-Dec Issue 6 Pg. 44-9 ISSN: 0372-9311 [Print] Russia (Federation)
PMID24605674 (Publication Type: Journal Article)
Chemical References
  • Adjuvants, Immunologic
  • Antigens, Bacterial
  • Diphtheria Toxin
  • Diphtheria-Tetanus-acellular Pertussis Vaccines
  • Tetanus Toxin
  • Aluminum Hydroxide
Topics
  • Adjuvants, Immunologic (adverse effects, pharmacology)
  • Aluminum Hydroxide (adverse effects, pharmacology)
  • Animals
  • Antigens, Bacterial (adverse effects, immunology, pharmacology)
  • Bordetella pertussis
  • Diphtheria Toxin (immunology, toxicity)
  • Diphtheria-Tetanus-acellular Pertussis Vaccines (adverse effects, immunology, pharmacology)
  • Drug Evaluation, Preclinical
  • Female
  • Guinea Pigs
  • Humans
  • Male
  • Meningoencephalitis (immunology, prevention & control)
  • Mice
  • Tetanus Toxin (immunology, toxicity)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: