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Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors.

AbstractINTRODUCTION:
Hepatitis C virus (HCV) infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA) and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies.
METHODS:
A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA) at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA) were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany), the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA), the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA) and line probe assay (LiPA - Siemens, Tarrytown, NY, USA) genotyping for HCV diagnosis.
RESULTS:
Of these new samples, 38.2% (39/102) were positive, 57.8% (59/102) were negative and 3.9% (4/102) were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102) of the samples. RIBA results were positive in 58.1% (25/43), negative in 9.3% (4/43) and indeterminate in 32.6% (14/43) of the samples. The prevailing genotypes were 1 (78.3%, 18/23), 3 (17.4%, 4/23) and 2 (4.3%, 1/23). All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL). Of these samples, 71.4% (10/14) were reevaluated six months later. Eighty percent (8/10) of these samples remained indeterminate by RIBA, and 20% (2/10) were negative.
CONCLUSIONS:
In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA.
AuthorsFelicidade Mota Pereira, Maria Alice Sant'ana Zarife, Eliana Almeida Gomes Reis, Mitermayer G Reis
JournalRevista da Sociedade Brasileira de Medicina Tropical (Rev Soc Bras Med Trop) 2014 Jan-Feb Vol. 47 Issue 1 Pg. 12-7 ISSN: 1678-9849 [Electronic] Brazil
PMID24603731 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Hepatitis C Antibodies
  • RNA, Viral
  • Recombinant Proteins
Topics
  • Adult
  • Blood Donors
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genotype
  • Hepacivirus (genetics, immunology)
  • Hepatitis C (diagnosis)
  • Hepatitis C Antibodies (blood)
  • Humans
  • Immunoblotting
  • Male
  • Polymerase Chain Reaction
  • RNA, Viral (blood)
  • Recombinant Proteins
  • Viral Load

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