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Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expression.

Abstract
Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is one of the most frequently mutated human tumor suppressor genes, implicated in cell growth and survival and suppressing tumor formation. Loss of PTEN activity, either at the protein or genomic level, has been related to many primary and metastatic malignancies including breast cancer. The present study investigates the heterozygosity, mutation spectrum and protein expression of PTEN in 43 patients with breast cancer or precursor lesions of the breast and 10 healthy individuals. Microsatellite analysis at the PTEN locus using D10S215, D10S541 and D10S579 markers indicated that the observed heterozygosity (Ho) is lower than the expected heterozygosity (Hs) in benign and malignant breast disease. Mutational analysis in exons 1, 5, 7 and 9 of the PTEN gene revealed several mutations, most of which cause truncation of the PTEN protein and consequently loss of activity. Increased circulating levels of PTEN and phosphorylated PTEN protein were also observed by immunostaining in patients with breast cancer and precursor breast lesions. In support, increased PTEN protein expression was detected in corresponding tissue specimens. Our data suggest an association between breast cancer and PTEN mutations, resulting in the production of truncated forms of the corresponding protein, thus indicating that breast carcinogenesis is potentially related to PTEN loss of activity rather than loss of expression. Peripheral blood sampling may provide an advantageous application for the determination of PTEN gene mutations and its protein expression in human cancer.
AuthorsPetros Kechagioglou, Rigini M Papi, Xeni Provatopoulou, Eleni Kalogera, Elli Papadimitriou, Petros Grigoropoulos, Aphroditi Nonni, George Zografos, Dimitrios A Kyriakidis, Antonia Gounaris
JournalAnticancer research (Anticancer Res) Vol. 34 Issue 3 Pg. 1387-400 (Mar 2014) ISSN: 1791-7530 [Electronic] Greece
PMID24596386 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • DNA, Neoplasm
  • PTEN Phosphohydrolase
  • PTEN protein, human
Topics
  • Biomarkers, Tumor (genetics, metabolism)
  • Breast (metabolism, pathology)
  • Breast Neoplasms (genetics, metabolism, pathology)
  • Carcinoma, Ductal, Breast (genetics, metabolism, pathology)
  • Carcinoma, Intraductal, Noninfiltrating (genetics, metabolism, pathology)
  • Carcinoma, Lobular (genetics, metabolism, pathology)
  • Case-Control Studies
  • Chromosomes, Human, Pair 10 (genetics)
  • DNA, Neoplasm (genetics)
  • Female
  • Humans
  • Immunoblotting
  • Immunoenzyme Techniques
  • Loss of Heterozygosity
  • Microsatellite Repeats
  • Mutation (genetics)
  • PTEN Phosphohydrolase (genetics, metabolism)
  • Phosphorylation
  • Polymorphism, Single-Stranded Conformational
  • Prognosis

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