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Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.

Abstract
In the previous study, we demonstrated that adipose-derived stem cells (ASCs) have neuroprotective effects against ischemic damage in the ventral horn of L5-6 levels at 3 days after ischemia/reperfusion. In the present study, we expanded our observations for 3 weeks after ischemia/reperfusion to rule out the possibility of delayed neuronal death in several days after ischemia/reperfusion. Transient spinal cord ischemia was induced by a 15 min aortic artery occlusion in the subrenal region and rabbit ASCs were administered intrathecally into recipient rabbits (2 × 10(5)) immediately after reperfusion. Transplantation of ASCs improved the neurological motor functions of the hindlimb 3 weeks after ischemia/reperfusion. Similarly, the cresyl violet-positive neurons were significantly increased at 3 weeks after ischemia/reperfusion compared to that in the vehicle (artificial cerebrospinal fluid)-treated group. The transplantation of ASCs significantly reduced reactive microglia induced by ischemia at 3 weeks after ischemia/reperfusion. In addition, transplantation of ASCs maintained the brain-derived neurotrophic factor (BDNF) levels 3 weeks after ischemia/reperfusion. These results suggest that the neuroprotective effects of ASCs are maintained 3 weeks after ischemia/reperfusion by modulating microgliosis and BDNF levels in the spinal cord.
AuthorsSeung Myung Moon, Woosuk Kim, Jin Young Chung, Wooseok Im, Dae Young Yoo, Hyo Young Jung, Moo-Ho Won, Jung Hoon Choi, In Koo Hwang
JournalBioMed research international (Biomed Res Int) Vol. 2014 Pg. 539051 ( 2014) ISSN: 2314-6141 [Electronic] United States
PMID24592394 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Brain-Derived Neurotrophic Factor
  • Neuroprotective Agents
  • BDNF protein, human
Topics
  • Adipose Tissue (cytology)
  • Animals
  • Aorta (pathology)
  • Brain-Derived Neurotrophic Factor (metabolism)
  • Ischemia (pathology, therapy)
  • Neuroprotective Agents
  • Rabbits
  • Reperfusion Injury (pathology, therapy)
  • Spinal Cord (pathology)
  • Stem Cell Transplantation
  • Stem Cells (cytology)

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