Despite being considered an emerging yeast related to immunocompromised individuals, severe
infections by Malassezia furfur have not been evaluated. During a one-year survey on yeasts
fungemia, 290 neonatal and 17 pediatric patients with intravascular
catheters,
lipid parenteral nutrition, prolonged ward stay, and surgery were enrolled. In addition, the origin of the
infection was investigated by swabbing hand skin of patients, parents, and healthcare workers and medical devices. All
biological specimens and swabs were cultured on Sabouraud
dextrose agar and Dixon
agar. The yeasts identification was based on morphological and biochemical features and by matrix-assisted
laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and confirmed by sequencing the internal transcribed spacer of nuclear
ribosomal DNA. A higher prevalence of M. furfur (2.1%) over Candida spp. (1.4%) caused
bloodstream infections (BSIs). Twelve
fungemia episodes were recorded: 2 by M. furfur in a pediatric ward and 10 in a neonatal intensive care unit (6 caused by M. furfur and 4 by Candida spp.). M. furfur was also isolated from the skin of all patients with BSIs, from the hand skin of a parent, and from an incubator surface and sheet. Patients with Candida spp. and M. furfur BSIs were successfully treated with intravenous
liposomal Amphotericin B. These findings highlight the need for a more accurate etiological diagnosis in high-risk patients by adding
lipid-supplemented
culture media for Malassezia in the current mycological routine as the clinical features, patient management, and outcomes in both Candida and Malassezia
fungemia do not differ.