Eosinophils from granulomas in murine schistosomiasis mansoni produce substance P.

Abstract	Granulomas are chronic, usually focal, tissue-destructive inflammatory reactions that usually form around slowly degradable, poorly soluble substances. They are dynamic lesions, regulated by complex immune mechanisms. Tachykinins are a family of neuropeptides characterized by the common C-terminal amino acid sequence -Phe-X-Gly-Leu-Met-NH2. One such tachykinin, substance P, has been reported to modulate immunologic responses. In this investigation, granulomas were examined for substance P. Granulomas were isolated from the livers of mice infected with murine schistosomiasis, and substance P was extracted. Immunoreactive substance P was detected by RIA. The authenticity of the molecule was confirmed by elution profile on HPLC. Immunoreactive substance P, identified by immunostaining, localized to eosinophils derived from collagenase-dispersed granulomas. Granulomas were then probed for expression of the gene for substance P (preprotachykinin). Preprotachykinin mRNA was localized to granuloma eosinophils by in situ oligonucleotide hybridization. It is concluded that substance P is present within the granuloma as a result of preprotachykinin production by eosinophils.
Authors	J V Weinstock, A Blum, J Walder, R Walder
Journal	Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 141 Issue 3 Pg. 961-6 (Aug 01 1988) ISSN: 0022-1767 [Print] United States
PMID	2456338 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Neuropeptides Protein Precursors RNA, Messenger Tachykinins Tissue Extracts preprotachykinin Substance P
Topics	Animals Chromatography, High Pressure Liquid Eosinophils (metabolism, pathology) Female Granuloma (metabolism, pathology) Immunohistochemistry Liver Diseases, Parasitic (metabolism, pathology) Mice Mice, Inbred CBA Neuropeptides (metabolism) Nucleic Acid Hybridization Protein Precursors (metabolism) RNA, Messenger (metabolism) Radioimmunoassay Schistosomiasis mansoni (metabolism, pathology) Substance P (biosynthesis, isolation & purification) Tachykinins Tissue Extracts (analysis)

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