Abstract | AIM: MATERIALS AND METHODS: DN was induced in Sprague-Dawley rats (150-200 g) by intraperitoneal administration of streptozotocin (STZ) (55 mg/kg, p.o.). Rats were divided into various groups, namely, STZ control (vehicle), hesperidin (25, 50, and 100 mg/kg, p.o.), insulin (10 IU/kg, s.c.), and combination of hesperidin (100 mg/kg, p.o.) with insulin (10 IU/kg, s.c.) for 4 weeks. Various behavioral ( allodynia and hyperalgesia), biochemical parameters [oxido-nitosative stress, Na-K- ATPase, aldose reductase (AR)], and molecular changes (TNF-α and IL-1β) along with hemodynamic changes were determined. RESULTS: Rats treated with hesperidin (50 and 100 mg/kg, p.o., 4 weeks) significantly reduced (p < 0.05) hyperglycemia and its metabolic abnormalities induced by intraperitoneal administration of STZ. The decreased nociceptive threshold, motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV), serum insulin as well as Na-K- ATPase activity were significantly increase (p < 0.05) by hesperidin (50 and 100 mg/kg, p.o.) treatment. It significantly attenuated (p < 0.05) elevated glycated hemoglobin, AR activity, oxido-nitrosative stress, neural calcium, and pro-inflammatory cytokines (TNF-α and IL-1β) levels. Histological aberration induced after STZ administration was restored by administration of hesperidin (50 and 100 mg/kg, p.o.) CONCLUSION:
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Authors | Asjad Visnagri, Amit D Kandhare, Shalendra Chakravarty, Pinaki Ghosh, Subhash L Bodhankar |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 52
Issue 7
Pg. 814-28
(Jul 2014)
ISSN: 1744-5116 [Electronic] England |
PMID | 24559476
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Glycated Hemoglobin A
- Insulin
- Interleukin-1beta
- Neuroprotective Agents
- Tumor Necrosis Factor-alpha
- Hesperidin
- Aldehyde Reductase
- Sodium-Potassium-Exchanging ATPase
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Topics |
- Aldehyde Reductase
(metabolism)
- Animals
- Biomarkers
(metabolism)
- Diabetic Neuropathies
(chemically induced, drug therapy)
- Dose-Response Relationship, Drug
- Drug Therapy, Combination
- Glycated Hemoglobin
(metabolism)
- Hesperidin
(pharmacology, therapeutic use)
- Hyperalgesia
(drug therapy)
- Hyperglycemia
(drug therapy)
- Insulin
(blood, pharmacology, therapeutic use)
- Interleukin-1beta
(metabolism)
- Neural Conduction
(drug effects, physiology)
- Neuralgia
(drug therapy)
- Neuroprotective Agents
(pharmacology)
- Oxidative Stress
(drug effects)
- Pain Measurement
- Rats
- Sciatic Nerve
(drug effects, metabolism, physiology)
- Sodium-Potassium-Exchanging ATPase
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
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