[Protective effects of hydrogen on renal ischemia/reperfusion injury in rats].

Abstract

OBJECTIVE:

To investigate the protective effects of hydrogen on renal ischemic/reperfusion injury and its mechanism in rats.

METHODS:

Eighteen adult male Sprague-Dawley rats were used and allocated into sham-operated group (Sham group), ischemic reperfusion group (I/R group) and hydrogen treated group (H2 group) randomly. Ischemic/reperfusion of the left renal pedicles following a right nephrectomy was investigated in I/R group. In the Sham group, the rats were subjected to identical surgical procedure without occlusion of left renal pedicles. In the H2 group, inhalation of the 2.5% hydrogen was initiated 10 min before reperfusion and last for 130 min till the end of the 120-min reperfusion. Plasma level of usea nitrogen (BUN), creatinine (Cr), maiondiaidehyde (MDA), the score of histopathological examination of left kidney were evaluated 24 h after operation in all subjects.

RESULTS:

Ischemia-reperfusion injury rat model was established successfully. Inhalation of hydrogen (2.5%) markedly reduced the level of serum BUN, CREA and MDA (P < 0.05). The histopathological outcome in the H2 group was significantly improved, compared to the I/R group (P < 0.05).

CONCLUSION:

Inhalation of hydrogen could attenuate renal ischemic/reperfusion injury in rats by decreasing the content of MDA.

• Authors: Kui Zeng, Han Huang, Xiao-Qin Jiang, Xiao-Jing Chen, Wei Huang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 45 Issue 1 Pg. 39-42 (Jan 2014) ISSN: 1672-173X [Print] China
• PMID: 24527579 (Publication Type: Journal Article)

Chemical References

• Malondialdehyde
• Hydrogen
• Creatinine

Topics

• Animals
• Blood Urea Nitrogen
• Creatinine (blood)
• Hydrogen (therapeutic use)
• Kidney (pathology)
• Male
• Malondialdehyde (blood)
• Nephrectomy
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (therapy)