Abstract | INTRODUCTION: METHODS: A novel serum ELISA was developed to measure serum NN and modified to measure serum nitrated albumin (NA). Levels of both NN and NA were measured in 397 samples from 49 patients with SLE followed through periods of disease flare and remission for a mean of 89 months. Anti- nucleosome antibody (anti-nuc) levels were measured in the same samples. The effects of 24 different clinical, demographic and serological variables on NN, NA and anti-nuc levels were assessed by univariable and multivariable analysis. RESULTS: Patients with SLE had higher mean NN than healthy controls or patients with other autoimmune rheumatic diseases (P =0.01). Serum samples from 18 out of 49 (36.7%) of SLE patients were never positive for NN. This group of 18 patients was characterized by lower anti- double stranded DNA antibodies (anti-dsDNA), disease activity and use of immunosuppressants. In the remaining 63.3%, NN levels were variable. High NN was significantly associated with anti-Sm antibodies, vasculitis, immunosuppressants, hydroxychloroquine and age at diagnosis. NN levels were raised in neuropsychiatric flares. NN levels did not completely parallel NA results, thus providing additional information over measuring nitration status alone. NN levels were not associated with anti-nuc levels. CONCLUSIONS: NN are raised in a subset of patients with SLE, particularly those who are anti-Sm positive. Elevated NN may be a marker of vascular activation and neuropsychiatric flares in these patients.
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Authors | Sara Croca, Paul Bassett, Charis Pericleous, Karim Fouad Alber, David Latchman, David Isenberg, Ian Giles, Anisur Rahman, Yiannis Ioannou |
Journal | Arthritis research & therapy
(Arthritis Res Ther)
Vol. 16
Issue 1
Pg. R48
(Feb 07 2014)
ISSN: 1478-6362 [Electronic] England |
PMID | 24502558
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Autoantigens
- Biomarkers
- Nucleosomes
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Topics |
- Adult
- Autoantibodies
(blood, immunology)
- Autoantigens
(immunology)
- Biomarkers
(blood)
- Cohort Studies
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Longitudinal Studies
- Lupus Erythematosus, Systemic
(blood, immunology)
- Male
- Nucleosomes
(immunology)
- Retrospective Studies
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