Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia.

Abstract

Hypoxia represents a major physiological challenge for prawn culture, and the hepatopancreas plays an important role in these processes. Here, we applied high-throughput sequencing technology to detect the gene expression profile of the hepatopancreas in Macrobrachium nipponense in response to hypoxia for 3 h and hypoxia for 24 h. Gene expression profiling identified 1925 genes that were significantly up- or down-regulated by dissolved oxygen availability. Functional categorization of the differentially expressed genes revealed that oxygen transport, electron transport chain, reactive oxygen species generation/scavenging, and immune response were the differentially regulated processes occurring during environmental hypoxia. Finally, quantitative real-time polymerase chain reaction using six genes independently verified the tag-mapped results. Immunohistochemistry analysis revealed, for the first time, hemocyanin protein expression as significant hypoxia-specific signature in prawns,which opens the way for in depth molecular studies of hypoxia exposure. The analysis of changes in hepatic gene expression in oriental river prawn provides a preliminary basis for a better understanding of the molecular response to hypoxia exposures.

• Authors: Shengming Sun, Fujun Xuan, Xianping Ge, Hongtuo Fu, Jian Zhu, Shiyong Zhang
• Journal: Gene (Gene) Vol. 534 Issue 2 Pg. 298-306 (Jan 25 2014) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 24498647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemocyanins
• Oxygen

Topics

• Animals
• Cluster Analysis
• Environment
• Hemocyanins (genetics)
• Hepatopancreas (metabolism, physiology)
• High-Throughput Screening Assays (methods)
• Hypoxia (metabolism)
• Oxygen (metabolism)
• Palaemonidae (genetics, metabolism)
• Transcriptome (genetics)