Kaiso interacts with p120-catenin to regulate β-catenin expression at the transcriptional level.

Abstract

BACKGROUND:

We have reported that p120-catenin could regulate β-catenin transcription in lung cancer cells, but the specific mechanism is unclear.

METHODS AND RESULTS:

In this study, bisulfite sequencing PCR showed that the β-catenin promoter region in SPC-A-1 and LTEP-a-2 lung cancer cell lines has Kaiso binding sites sequences and CpG islands which may combine with Kaiso. The demethylating reagent 5-Aza-2'-deoxycytidine significantly upregulated β-catenin mRNA expression in lung cancer cell lines, whereas expression was significantly reduced following transfection with Kaiso. However, the upregulation of β-catenin mRNA expression after treatment with 5-Aza-2'-deoxycytidine was not reduced by subsequent transfection with Kaiso cDNA. Chromatin immunoprecipitation showed that, in lung cancer cell lines, methylated CpG-dinucleotides sequences combined with Kaiso and the Kaiso binding sites sequence did not. The capacity of Kaiso to combine with p120-catenin isoforms was confirmed by immunoprecipitation.

CONCLUSIONS:

Based on these results, we concluded that Kaiso participates in the regulation by p120ctn of β-catenin mRNA expression in the lung cancer cell lines.

• Authors: Yang Liu, Qian-Ze Dong, Si Wang, Hong-Tao Xu, Yuan Miao, Liang Wang, En-Hua Wang
• Journal: PloS one (PLoS One) Vol. 9 Issue 2 Pg. e87537 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24498333 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Catenins
• Enzyme Inhibitors
• Transcription Factors
• ZBTB33 protein, human
• beta Catenin
• Decitabine
• Azacitidine
• Delta Catenin
• CTNND1 protein, human

Topics

• Azacitidine (analogs & derivatives, pharmacology)
• Blotting, Western
• Catenins (genetics, metabolism)
• Cell Line, Tumor
• CpG Islands (genetics)
• DNA Methylation
• Decitabine
• Enzyme Inhibitors (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• Promoter Regions, Genetic (genetics)
• Protein Binding
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription Factors (genetics, metabolism)
• Transcription, Genetic
• Up-Regulation (drug effects)
• beta Catenin (genetics, metabolism)
• Delta Catenin