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[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].

Abstract
Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.
AuthorsG Ignatov, A Tunkara, M Ignatova
JournalVeterinarno-meditsinski nauki (Vet Med Nauki) Vol. 24 Issue 8 Pg. 44-7 ( 1987) ISSN: 0324-1068 [Print] Bulgaria
Vernacular TitleIzpolzuvane na gama-globulini ot peritonealna technost ot kotki, bolni ot infektsiozen peritonit (FIP), za imunofluorestsentno pozitivirane na TGE-antigen.
PMID2449762 (Publication Type: Comparative Study, English Abstract, Journal Article)
Chemical References
  • Antigens, Viral
  • Fluoresceins
  • Fluorescent Dyes
  • Immunoglobulin G
  • Thiocyanates
  • gamma-Globulins
  • Fluorescein-5-isothiocyanate
Topics
  • Animals
  • Antigens, Viral (analysis)
  • Ascitic Fluid (immunology)
  • Cat Diseases (immunology)
  • Cats
  • Coronaviridae (immunology)
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Fluorescent Antibody Technique (veterinary)
  • Fluorescent Dyes
  • Gastroenteritis, Transmissible, of Swine (immunology)
  • Immunoglobulin G (isolation & purification)
  • Intestine, Small (immunology)
  • Peritonitis (immunology, veterinary)
  • Swine
  • Thiocyanates
  • Transmissible gastroenteritis virus (immunology)
  • gamma-Globulins (isolation & purification)

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