Abstract |
Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG- globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.
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Authors | G Ignatov, A Tunkara, M Ignatova |
Journal | Veterinarno-meditsinski nauki
(Vet Med Nauki)
Vol. 24
Issue 8
Pg. 44-7
( 1987)
ISSN: 0324-1068 [Print] Bulgaria |
Vernacular Title | Izpolzuvane na gama-globulini ot peritonealna technost ot kotki, bolni ot infektsiozen peritonit (FIP), za imunofluorestsentno pozitivirane na TGE-antigen. |
PMID | 2449762
(Publication Type: Comparative Study, English Abstract, Journal Article)
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Chemical References |
- Antigens, Viral
- Fluoresceins
- Fluorescent Dyes
- Immunoglobulin G
- Thiocyanates
- gamma-Globulins
- Fluorescein-5-isothiocyanate
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Topics |
- Animals
- Antigens, Viral
(analysis)
- Ascitic Fluid
(immunology)
- Cat Diseases
(immunology)
- Cats
- Coronaviridae
(immunology)
- Fluorescein-5-isothiocyanate
- Fluoresceins
- Fluorescent Antibody Technique
(veterinary)
- Fluorescent Dyes
- Gastroenteritis, Transmissible, of Swine
(immunology)
- Immunoglobulin G
(isolation & purification)
- Intestine, Small
(immunology)
- Peritonitis
(immunology, veterinary)
- Swine
- Thiocyanates
- Transmissible gastroenteritis virus
(immunology)
- gamma-Globulins
(isolation & purification)
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