3-dimensional tissue is formed from cancer cells in vitro on Gelfoam®, but not on Matrigel™.

Abstract

Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam(®), a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of α(v) integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam(®), the cells formed three-dimensional tissue-like structures. The behavior of 143B osteosarcoma cells on Gelfoam(®) in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue-like structures were observed only in Gelfoam(®) culture. The data in this report suggest a flexible structural substrate such as Gelfoam(®) provides a more in vivo-like culture condition than monolayer culture or Matrigel(TM) and that Matrigel(TM) does not result in actual three-dimensional culture.

• Authors: Yasunori Tome, Fuminari Uehara, Sumiyuki Mii, Shuya Yano, Lei Zhang, Naotoshi Sugimoto, Hiroki Maehara, Michael Bouvet, Hiroyuki Tsuchiya, Fuminori Kanaya, Robert M Hoffman
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 115 Issue 8 Pg. 1362-7 (Aug 2014) ISSN: 1097-4644 [Electronic] United States
• PMID: 24497277 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2014 Wiley Periodicals, Inc.

Chemical References

• Drug Combinations
• Integrin alpha Chains
• Laminin
• Proteoglycans
• matrigel
• Green Fluorescent Proteins
• Collagen

Topics

• Bone Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Collagen (chemistry)
• Drug Combinations
• Gelatin Sponge, Absorbable (chemistry)
• Green Fluorescent Proteins
• Humans
• In Vitro Techniques
• Integrin alpha Chains (biosynthesis)
• Laminin (chemistry)
• Osteosarcoma (metabolism, pathology)
• Proteoglycans (chemistry)