To establish cardiomyocyte
hypoxia/reoxygenation injury model by culturing primary cardiomyocytes from suckling SD rats, in order to study the effect of
succinic acid on LDH leakage rate cardiomyocyte
ischemia/reperfusion injury. Furthermore, flow cytometry and western blot were conducted to detect the effect of
succinic acid on cardiomyocyte apoptosis, cleaved
caspase-3 and p-Akt, and discuss the protective effect of
succinic acid on primary cardiomyocyte
hypoxia/reoxygenation injury of primary cardiomyocytes from neonatal SD rats. According to the findings of the study,
succinic acid at the concentrations ranging between 31.25 mg x L(-1) and 500 mg x L(-1) had no significant effect on primary cardiomyocyte activity, and
succinic acid at the concentrations of 400, 200, 100, 50 mg x L(-1) could notably reduce cardiomyocyte
ischemia/reperfusion LDH leakage rate (P < 0.01 or P < 0.05, respectively).
Succinic acid at the concentrations of 400 mg x L(-1) and 200 mg x L(-1) could significantly reduce the percentage of cardiomyocyte apoptosis (P < 0.05), and inhibit the
protein expression of cleaved
caspase-3 caused by cardiomyocyte
ischemia/reperfusion (P < 0.05).
Succinic acid at the concentration of 400 mg x L(-1) could remarkably increase the
protein expression of cardiomyocyte Akt (P < 0.05), while
succinic acid at the concentration of 200 mg x L(-1) had no obvious effect on the
protein expression of cardiomyocyte Akt. Therefore, this study demonstrated that
succinic acid could inhibit
necrosis and apoptosis caused by cardiomyocyte
hypoxia/reoxygenation by activating Akt phosphorylation.