Passive protective effect of chicken egg yolk immunoglobulins against experimental Vibrio anguillarum infection in ayu (Plecoglossus altivelis).

Abstract

Oral administration of chicken egg yolk immunoglobulins (IgY) has attracted much attention as a means for controlling infectious diseases caused by microorganisms. This study evaluated the protective effect of IgY against Vibrio anguillarum infection in ayu, Plecoglossus altivelis. IgY was isolated from egg yolks laid by hens initially immunized with formalin-inactivated V. anguillarum. Lower mortality of ayu was observed in groups treated with anti-V. anguillarum IgY (aVIgY), compared with those treated with saline or with nonspecific IgY (nspIgY). All fish in saline-treated groups died within seven days after bacterial inoculation. The bacterial load in blood, liver, and spleen was significantly lower in fish treated with aVIgY than in fish treated with nspIgY. aVIgY treatment significantly reduced tumor necrosis factor-α (PaTNF-α), interleukin-1β (PaIL-1β), transforming growth factor-β (PaTGF-β), and leukocyte cell-derived chemotaxin-2 (PaLECT2) transcript levels in the head kidney, spleen, and liver of ayu challenged by V. anguillarum, compared with nspIgY treatment. The phagocytic activity of macrophages for V. anguillarum in the presence of specific IgY was significantly higher than that seen for nonspecific IgY. These results suggest that passive immunization by oral intubation with pathogen-specific IgY may provide a valuable treatment for V. anguillarum infection in ayu.

• Authors: Chang-Hong Li, Xin-Jiang Lu, Deng-Feng Li, Jiong Chen
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 37 Issue 1 Pg. 108-14 (Mar 2014) ISSN: 1095-9947 [Electronic] England
• PMID: 24486630 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• DNA Primers
• Egg Proteins
• Immunoglobulins

Topics

• Administration, Oral
• Analysis of Variance
• Animals
• Chickens
• Cytokines (metabolism)
• DNA Primers (genetics)
• Egg Proteins (administration & dosage, immunology, pharmacology)
• Fish Diseases (immunology, microbiology)
• Gene Expression Regulation (drug effects, immunology)
• Immunoglobulins (administration & dosage, immunology, pharmacology)
• Kaplan-Meier Estimate
• Macrophages (drug effects, immunology)
• Osmeriformes
• Phagocytosis (drug effects, immunology)
• Real-Time Polymerase Chain Reaction (veterinary)
• Vibrio (immunology)
• Vibrio Infections (immunology, veterinary)