Arginine kinase of Litopenaeus vannamei involved in white spot syndrome virus infection.

Abstract

Virus-host interaction is important for virus infection. White spot syndrome virus VP14 contains transmembrane and signal peptides domain, which is considered to be important for virus infection. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP14 with host cell. A new shrimp protein (arginine kinase of Litopenaeus vannamei, LvAK) is selected and its localization in shrimp cells is also confirmed. Cellular localization of LvAK protein in shrimp hemocytes showed that LvAK was primarily located at the periphery of hemocytes and was scarcely detectable in the nucleus. Tissue distribution indicated that arginine kinase gene was spread commonly in the tissues and was highly present in shrimp muscle tissue. The expression of LvAK mRNA in muscle was significantly up-regulated after WSSV stimulation. Indirect immunofluorescence assay showed that LvAK interacted with VP14 in WSSV-infected shrimp. Injection of LvAK protein enhanced the mortality of shrimp infected with white spot syndrome virus (WSSV). These results showed that LvAK is involved in WSSV infection. Future research on this topic will help to reveal the molecular mechanism of WSSV infection.

• Authors: Fang-fang Ma, Qing-hui Liu, Guang-kuo Guan, Chen Li, Jie Huang
• Journal: Gene (Gene) Vol. 539 Issue 1 Pg. 99-106 (Apr 10 2014) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 24486504 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• RNA, Messenger
• RNA, Small Interfering
• Recombinant Proteins
• Viral Fusion Proteins
• Arginine Kinase

Topics

• Animals
• Arginine Kinase (genetics, metabolism)
• Fluorescent Antibody Technique, Indirect
• Hemocytes (enzymology)
• Host-Pathogen Interactions
• Muscles (enzymology, metabolism)
• Penaeidae (enzymology, immunology, virology)
• RNA Interference
• RNA, Messenger (biosynthesis)
• RNA, Small Interfering
• Recombinant Proteins (genetics, metabolism)
• Sequence Analysis, DNA
• Tissue Distribution
• Viral Fusion Proteins (genetics, metabolism)
• White spot syndrome virus 1 (genetics, pathogenicity)