HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Identification of proteins with increased levels in ameloblastic carcinoma.

AbstractPURPOSE:
The comparative proteomic approach by a combination of 2-dimensional electrophoresis and matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MS) analysis is an attractive strategy for the discovery of cancer biomarkers and therapeutic targets. The identification of protein biomarkers associated with ameloblastic carcinoma (AC), a malignant epithelial odontogenic tumor, will potentially improve the diagnostic and prognostic accuracy for this malignant neoplasm. The aim of the present study was to identify highly expressed proteins in AC that could be considered as potential biomarkers.
MATERIALS AND METHODS:
The protein profile of an AC was compared with the protein profiles of 3 cases of benign ameloblastoma. Proteins that showed increased levels in AC were identified using MS, and the augmented amount of some of these proteins in the malignant lesion was confirmed by Western blot or immunohistochemistry.
RESULTS:
We detected a total of 782 spots in the protein profile of AC, and 19 of them, showing elevated levels compared with benign ameloblastoma, were identified using MS. These proteins have been implicated in several cellular functions, such as cell structure, metabolism, stress response, and signal transduction.
CONCLUSIONS:
The increased expression of the identified proteins and the minor expression of some proteins that might inhibit tumor progression could be involved in the evolution from a benign lesion to carcinoma.
AuthorsAlejandro García-Muñoz, Ronell Bologna-Molina, Beatriz Aldape-Barrios, Carlos Licéaga-Escalera, Luis A Montoya-Pérez, Mario A Rodríguez
JournalJournal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (J Oral Maxillofac Surg) Vol. 72 Issue 6 Pg. 1183-96 (Jun 2014) ISSN: 1531-5053 [Electronic] United States
PMID24485979 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.
Chemical References
  • Biomarkers, Tumor
  • Chromatin
  • HSP70 Heat-Shock Proteins
  • Keratin-19
  • Neoplasm Proteins
  • Proteome
  • Collagen
  • Glyceraldehyde-3-Phosphate Dehydrogenases
Topics
  • Ameloblastoma (pathology)
  • Ameloblasts (pathology)
  • Biomarkers, Tumor (analysis)
  • Blotting, Western
  • Cell Nucleus (pathology)
  • Cell Polarity
  • Chromatin (pathology)
  • Collagen
  • Electrophoresis, Gel, Two-Dimensional (methods)
  • Electrophoresis, Polyacrylamide Gel (methods)
  • Epithelial Cells (pathology)
  • Epithelium (pathology)
  • Glyceraldehyde-3-Phosphate Dehydrogenases (analysis)
  • HSP70 Heat-Shock Proteins (analysis)
  • Humans
  • Immunohistochemistry
  • Isoelectric Focusing (methods)
  • Keratin-19 (analysis)
  • Neoplasm Proteins (analysis)
  • Odontogenic Tumors (pathology)
  • Peptide Mapping (methods)
  • Proteome (analysis)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: