Effects of DNA damage and short-term spindle disruption on oocyte meiotic maturation.

DNA damage has recently been shown to inhibit or delay germinal vesicle breakdown (GVBD) in mouse oocytes, but once meiosis resumes, DNA-damaged oocytes are able to extrude the first polar body. In this study, using porcine oocytes, we showed that DNA damage did not affect GVBD, but inhibited the final stages of maturation, as indicated by failure of polar body emission. Unlike mitotic cells in which chromosome mis-segregation causes DNA double-strand breaks, meiotic mouse oocytes did not show increased DNA damage after disruption of chromosome attachment to spindle microtubules. Nocodazole-treated oocytes did not display increased DNA damage signals that were marked by γH2A.X signal strength, but reformed spindles and underwent maturation, although aneuploidy increased after extended nocodazole treatment. By using the mouse for parthenogenetic activation studies, we showed that early cleavage stage embryos derived from parthenogenetic activation of nocodazole-treated oocytes displayed normal activation rate and normal γH2A.X signal strength, indicating that no additional DNA damage occured. Our results suggest that DNA damage inhibits porcine oocyte maturation, while nocodazole-induced dissociation between chromosomes and microtubules does not lead to increased DNA damage either in mouse meiotic oocytes or in porcine oocytes.
AuthorsT Zhang, G L Zhang, J Y Ma, S T Qi, Z B Wang, Z W Wang, Y B Luo, Z Z Jiang, H Schatten, Q Y Sun
JournalHistochemistry and cell biology (Histochem Cell Biol) Vol. 142 Issue 2 Pg. 185-94 (Aug 2014) ISSN: 1432-119X [Electronic] Germany
PMID24477549 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Aneugens
  • Antibiotics, Antineoplastic
  • Histones
  • Tubulin Modulators
  • gamma-H2AX protein, mouse
  • Bleomycin
  • Nocodazole
  • Aneugens (pharmacology)
  • Aneuploidy
  • Animals
  • Antibiotics, Antineoplastic (pharmacology)
  • Bleomycin (pharmacology)
  • Chromosomes (genetics)
  • DNA Breaks, Double-Stranded
  • Female
  • Histones (metabolism)
  • Meiosis (genetics)
  • Mice
  • Mice, Inbred ICR
  • Microtubules (genetics)
  • Nocodazole (pharmacology)
  • Oocytes (cytology)
  • Ovarian Follicle (cytology)
  • Parthenogenesis (genetics)
  • Spindle Apparatus (genetics)
  • Swine
  • Tubulin Modulators (pharmacology)

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