Abstract |
The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism's response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.
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Authors | Ievgen Motorykin, Maret G Traber, Robert L Tanguay, Claudia S Maier |
Journal | Journal of proteome research
(J Proteome Res)
Vol. 13
Issue 3
Pg. 1647-56
(Mar 07 2014)
ISSN: 1535-3907 [Electronic] United States |
PMID | 24476500
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Proteome
- Tissue Extracts
- Vitamin E
- Ascorbic Acid
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Topics |
- Adaptation, Physiological
- Animals
- Ascorbic Acid
(administration & dosage, metabolism)
- Ascorbic Acid Deficiency
(metabolism)
- Chromatography, Liquid
- Humans
- Mass Spectrometry
(methods)
- Metabolic Networks and Pathways
- Protein Interaction Mapping
- Proteome
(analysis, metabolism)
- Tissue Extracts
(chemistry)
- Vitamin E
(administration & dosage, metabolism)
- Vitamin E Deficiency
(metabolism)
- Zebrafish
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