Abstract | INTRODUCTION: METHODS:
HPMA copolymers containing 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid ( DOTA) were synthesized by reversible addition-fragmentation transfer (RAFT) copolymerization and subsequently labeled with either (111)In for imaging or (90)Y for efficacy studies. Radiolabel stability was characterized in vitro with mouse serum. Imaging and efficacy studies were conducted in DU145 prostate tumor bearing mice. Imaging was performed using single photon emission computerized tomography (SPECT). Localized mild tumor hyperthermia was achieved by plasmonic photothermal therapy using gold nanorods. RESULTS:
HPMA copolymer- DOTA conjugates demonstrated efficient labeling and stability for both radionuclides. Imaging analysis showed a marked increase of radiolabeled copolymer within the hyperthermia treated prostate tumors, with no significant accumulation in non-targeted tissues. The greatest reduction in tumor growth was observed in the hyperthermia treated tumors with (90)Y HPMA copolymer conjugates. Histological analysis confirmed treatment efficacy and safety. CONCLUSION:
HPMA copolymer- DOTA conjugates radiolabeled with both the imaging and treatment radioisotopes, when combined with hyperthermia can serve as an image guided approach for efficacious treatment of prostate tumors.
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Authors | Brandon Buckway, Nick Frazier, Adam J Gormley, Abhijit Ray, Hamidreza Ghandehari |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 41
Issue 3
Pg. 282-9
(Mar 2014)
ISSN: 1872-9614 [Electronic] United States |
PMID | 24461626
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- Acrylamides
- Heterocyclic Compounds, 1-Ring
- Yttrium Radioisotopes
- 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid
- Gold
- N-(2-hydroxypropyl)methacrylamide
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Topics |
- Acrylamides
(chemistry, pharmacokinetics, therapeutic use)
- Animals
- Cell Line, Tumor
- Female
- Gold
(chemistry, therapeutic use)
- Heterocyclic Compounds, 1-Ring
(chemistry)
- Humans
- Hyperthermia, Induced
- Male
- Mice
- Nanotubes
- Prostatic Neoplasms
(diagnostic imaging, pathology, radiotherapy)
- Tomography, Emission-Computed, Single-Photon
- Yttrium Radioisotopes
(therapeutic use)
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