The aim of this study was to evaluate the
antioxidant activities of
diethyl ether (DEE) and
methanol (M) extracts from brown alga Padina boergesenii using in vitro and in vivo
antioxidant assay, which may help to relate the
antioxidant properties with the possible outline of its ameliorative effect. M extract showed higher radical scavenging activity through ferric reducing
antioxidant power 139.11 µmol
tannic acid equivalent/g; DPPH 71.32 ± 0.56%;
deoxyribose radical 88.31 ± 0.47%, and total
antioxidant activity 0.47 ± 0.02 mg
ascorbic acid equivalents/g. Oxidative red blood cell (RBC)
hemolysis inhibition rate was significantly higher in M extract (150 mg/kg
body weight) in reference to total phenolic content (r = 0.935). Rats administered with DEE and M extracts (150 mg/kg
body weight) for seven days before the administration of
ferric nitrilotriacetate (9 mg of Fe/mg/kg bodyweight). Rats pretreated with extracts significantly changed the level of renal microsomal lipid peroxidation,
glutathione, and
antioxidant enzymes in post-mitochondrial supernatant (P < 0.05). Ameliorative effect of extracts against renal oxidative damage was evident in rat kidney through changes in necrotic and epithelial cells. HPTLC technique has identified the presence of
rutin with reference to retardation factor (Rf ) in both the extracts. These findings support the source of
polyphenols (
rutin) from P. boergesenii had potent
antioxidant activity; further work on isolation of bioactive compounds can be channeled to develop as a natural
antioxidant.