Effects of mesenchymal stem cells transplanted at different time points in a rat remnant kidney model.

Abstract

AIMS:

The optimal time for mesenchymal stem cell (MSCs) transplantation remains an unresolved issue. We compared the effects of MSCs on a rat remnant kidney model.

METHODS:

Male Sprague-Dawley rats were randomly divided and treated with a corresponding reagent at 4, 8, 12 and 16 weeks, respectively. A remnant kidney model was established and MSCs were injected into rats. The migration of MSCs was then assessed by using cell-tracking experiments. Renal function and histological analyses were performed 4 weeks after MSC transplantation. Immunohistochemistry, Western blotting and real-time polymerase chain reaction were used to detect the TGF-β1 and α-SMA levels.

RESULTS:

Four weeks after MSC injection, MSCs were found to migrate to the injured kidney. Significant histological damage improvement was observed after the treatment of MSCs at 4 and 8 weeks. The functional benefits of MSC treatment were observed in the 5/6 nephrectomy (Nx) + MSC group and the benefits were significantly higher at 4 and 8 weeks than at other time points (p < 0.05). Meanwhile, serum creatinine and urea levels as well as glomerular sclerosis and tubulointerstitial injury indexes were decreased at 4 and 8 weeks. Compared with the 5/6 Nx + PBS group, TGF-β1 and α-SMA levels were decreased in the 5/6 Nx + MSC group.

CONCLUSION:

These data can be used to optimize the MSC transplantation time point as a therapeutic modality.

• Authors: Xiaohui Bian, Beiru Zhang, Wei Guo, Na Liu, Yu Bai, Jianing Miao, Guifeng Zhao, Bo Liu, Shubao Wang, Li Ma, Liqiang Zheng, Yueyang Zhao, Detian Li, Jiangmin Feng
• Journal: American journal of nephrology (Am J Nephrol) Vol. 39 Issue 1 Pg. 75-84 ( 2014) ISSN: 1421-9670 [Electronic] Switzerland
• PMID: 24457259 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Transforming Growth Factor beta
• smooth muscle actin, rat
• Urea
• Creatinine

Topics

• Actins (metabolism)
• Animals
• Cell Movement
• Creatinine (blood)
• Fibrosis (therapy)
• Glomerular Filtration Rate
• Glomerulosclerosis, Focal Segmental (blood)
• Kidney Transplantation (methods)
• Kidney Tubules (injuries)
• Male
• Mesenchymal Stem Cells (cytology)
• Rats
• Rats, Sprague-Dawley
• Time Factors
• Transforming Growth Factor beta (metabolism)
• Urea (blood)