Hypertonic HBOC-201 decreases neutrophil activation after hemorrhagic shock.

Abstract

OBJECTIVE:

To evaluate neutrophil activation after exposure to standard HBC-201 (suspended in lactate Ringer's solution) versus HBOC-201 suspended in hypertonic 7.5% saline solution.

METHODS:

We use plasma and tissue obtained from pigs subjected to controlled hemorrhagic shock and an ex vivo model of stimulated human whole blood. The pigs were resuscitated with the following (n = 8 per group) standard HBOC-201, or hypertonic HBOC-201. We used HTS 7.5%, Ringer's lactate as control resuscitation. Human blood was stimulated with same fluids. We measured the following neutrophil markers; IL-8, H2O2 in pig plasma, MPO in pig tissue, and H2O2, IL-8, and CD11b/CD18 in human whole blood.

RESULTS:

H2O2 and IL-8 as well as tissue MPO were significantly decreased in pigs resuscitated with HT-HBOC-201 and HT 7.5%. Ex vivo experiments blood diluted with HTS and HT-HBOC-201 revealed lower expression of CD11b/CD18, H2O2, and IL-8. Blood diluted with HBOC-201 had a higher CD11b/CD18 expression than blood diluted with LR solution.

CONCLUSION:

Our in vivo and ex vivo experiments indicate that HBOC-201 suspended in hypertonic 7.5% saline solution is associated with significantly less neutrophil activation when compared to standard HBOC-201 suspended in lactate Ringer's solution.

• Authors: Fernando A Rivera-Chávez, Amanda Lu, Ming-Mei Liu, Adil Abdalla, Joseph P Minei
• Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (J Invest Surg) Vol. 27 Issue 1 Pg. 14-20 (Feb 2014) ISSN: 1521-0553 [Electronic] United States
• PMID: 24456255 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Substitutes
• CD11b Antigen
• CD18 Antigens
• Hemoglobins
• Interleukin-8
• Saline Solution, Hypertonic
• HBOC 201
• Hydrogen Peroxide
• Peroxidase

Topics

• Animals
• Blood Substitutes (pharmacology)
• CD11b Antigen (blood)
• CD18 Antigens (blood)
• Disease Models, Animal
• Hemoglobins (pharmacology)
• Humans
• Hydrogen Peroxide (blood)
• Interleukin-8 (blood)
• Neutrophil Activation (drug effects, physiology)
• Neutrophils (drug effects, physiology)
• Peroxidase (metabolism)
• Saline Solution, Hypertonic
• Shock, Hemorrhagic (metabolism, physiopathology)
• Swine