Abstract | BACKGROUND:
Sparganosis is caused by the invasion of Spirometra sparganum into various tissues/organs. Subcutaneous sparganosis can be diagnosed by biopsy, while visceral/cerebral sparganosis is not easy to be diagnosed. The diagnosis depends largely on the detection of specific anti-sparganum antibodies. The specificity of the ELISA could be increased by using S. mansoni sparganum excretory-secretory (ES) antigens, but it also had the cross-reactions with sera of patients with cysticercosis or paragonimiasis. The aim of this study was to identify early specific diagnostic antigens in S. mansoni sparganum ES proteins. METHODS: The sparganum ES proteins were analyzed by two-dimensional electrophoresis (2-DE) and Western blot probed with early sera from infected mice at 14 days post- infection. The immunoreactive protein spots were characterized by MALDI-TOF/ TOF-MS. RESULTS: A total of approximately 149 proteins spots were detected with isoelectric point (pI) varying from 3 to 7.5 and molecular weight from 20 to 115 kDa and seven protein spots with molecular weight of 23-31 kDa were recognized by the infection sera. Three of seven spots were successfully identified and characterized as the same S. mansoni protein ( cysteine protease), and the proteins of other 4 spots were not included in the databases. CONCLUSION:
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Authors | Dan Dan Hu, Jing Cui, Li Wang, Li Na Liu, Tong Wei, Zhong Quan Wang |
Journal | Iranian journal of parasitology
(Iran J Parasitol)
Vol. 8
Issue 3
Pg. 408-16
(Jul 2013)
ISSN: 1735-7020 [Print] Iran |
PMID | 24454434
(Publication Type: Journal Article)
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