The aim of this study was to evaluate bone repair in anemic and non-anemic rats submitted or not to
laser phototherapy and
hydroxyapatite graft. Animals were divided in eight groups of five animals: Clot;
Laser; Graft; Graft +
Laser;
iron deficiency anemia (IDA) + Clot; IDA +
Laser; IDA + graft; IDA + graft +
Laser. When appropriate irradiation with infrared
laser was done during 15 days at a 48-h interval. Animals were killed at day 30; samples were analyzed by Raman spectroscopy. Three shifts were studied and statistically analyzed: ~960, ~1,070, and ~1,454 cm(-1). Graft +
laser showed highest ~960 peak was statistically different from all other healthy groups. No statistical difference was found between Clot and IDA + Clot in any shift. The IDA + Graft and IDA + Graft +
Laser groups had low mean peak values for shifts ~960, ~1,070, and ~1,454 cm(-1). The results in this study indicate that using
hydroxyapatite (HA) and
laser irradiation in healthy subjects is favorable to
mineral deposition and bone maturation, this being of importance for some groups at risk, such as astronauts. In
iron deficiency anemia cases, the use of graft, associated or not to
laser irradiation, resulted in low
collagen and low
carbonate and
phosphate HA.