Significant increase of prostaglandin E-major urinary metabolite in male smokers: a screening study of age and gender differences using a simple radioimmunoassay.

Abstract

BACKGROUND:

For the assessment of inflammatory status, we have developed a simple, reliable radioimmunoassay (RIA) of prostaglandin E-major urinary metabolite (PGE-MUM), which remains stable in urine after it is metabolized. Using this method, we conducted a screening study to compare standard values of PGE-MUM to serum C-reactive protein (CRP) levels in health check volunteers.

METHODS:

PGE-MUM (micrograms per gram creatinine) was measured in normal urine samples obtained from 797 samples in volunteers for health check, using a newly developed RIA, and analyzed in relation to age, gender, smoking, and drinking habits. Results were compared to serum CRP.

RESULTS:

PGE-MUM was significantly higher in males than in females. It was significantly higher in smoking males, compared to males who had never smoked (nonsmokers), particularly in those above 40 years of age. In nonsmokers, PGE-MUM declined in males with advancing age, while it rose in females. Although PGE-MUM reflected current smoking status, independent of smoking index (SI), serum CRP indicated both current and former smoking condition, rather dependent upon SI.

CONCLUSIONS:

PGE-MUM increases in smokers, as suggested by possible inflammatory injury of pulmonary tissue. This RIA method for PGE-MUM may be thus a sensitive and reliable biomarker for current inflammation, different from serum CRP.

• Authors: Isao Okayasu, Hiromitsu Ohnishi, Istvan Sarandi, Junko Shojima, Junko Komatsu, Masae Oritsu, Masataka Sasabe, Kaori Okayasu Nanami, Masaaki Matsuura, Jun-Ichi Azumi, Satoru Ito, Mutsunori Fujiwara
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 28 Issue 1 Pg. 32-41 (Jan 2014) ISSN: 1098-2825 [Electronic] United States
• PMID: 24375858 (Publication Type: Journal Article)
• Copyright: © 2013 Wiley Periodicals, Inc.

Chemical References

• Prostaglandins E
• C-Reactive Protein

Topics

• Adult
• Age Factors
• C-Reactive Protein (urine)
• Chromatography, High Pressure Liquid
• Drinking Behavior
• Female
• Healthy Volunteers
• Humans
• Male
• Mass Screening
• Middle Aged
• Prostaglandins E (urine)
• Radioimmunoassay (methods)
• Radioisotope Dilution Technique
• Reproducibility of Results
• Sex Characteristics
• Smoking (urine)