Activity of imidazole compounds on Leishmania (L.) infantum chagasi: reactive oxygen species induced by econazole.

Abstract

Drug repositioning has been considered a promising approach to discover novel treatments against neglected diseases. Among the major protozoan diseases, leishmaniasis remains a public health threat with few therapeutic alternatives, affecting 12 million people in 98 countries. In this study, we report the in vitro antileishmanial activity of the imidazole drugs clotrimazole, and for the first time in literature, econazole and bifonazole and their potential action to affect the regulation of reactive oxygen species (ROS) of the parasites. The lethal action of the imidazoles was investigated using spectrofluorimetric techniques to detect ROS content, plasma membrane permeability, and mitochondrial membrane potential. The imidazoles showed activity against L. (L.) infantum chagasi promastigotes with IC50 values in a range of 2-8 μM; econazole was also effective against Leishmania intracellular amastigotes, with an IC50 value of 11 μM, a similar in vitro effectiveness to miltefosine. Leishmania promastigotes rapidly up-regulated the ROS release after incubation with the imidazoles, but econazole showed a marked increase in ROS content of approximately 1,900 % higher than untreated parasites. When using SYTOX(®) Green as a fluorescent probe, the imidazoles demonstrated considerable interference in plasma membrane permeability at the early time of incubation; econazole resulted in the higher influx of SYTOX(®) Green at 60 min. Despite cellular alterations, no depolarization could be observed to the mitochondrial membrane potential of Leishmania until 60 min. The lethal action of econazole involved strong permeabilization of plasma membrane of promastigotes, with an overloaded ROS content that contributed to the death of parasites. Affecting the ROS regulation of Leishmania via small molecules would be an interesting strategy for new drugs.

• Authors: Juliana Tonini Mesquita, Thais Alves da Costa-Silva, Samanta Etel Treiger Borborema, André Gustavo Tempone
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 389 Issue 1-2 Pg. 293-300 (Apr 2014) ISSN: 1573-4919 [Electronic] Netherlands
• PMID: 24374794 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiprotozoal Agents
• Imidazoles
• Reactive Oxygen Species
• Econazole
• imidazole
• Clotrimazole
• bifonazole

Topics

• Antiprotozoal Agents (pharmacology)
• Cell Membrane (drug effects, metabolism)
• Cell Membrane Permeability (drug effects)
• Clotrimazole (pharmacology)
• Econazole (pharmacology)
• Imidazoles (pharmacology)
• Leishmania (drug effects, metabolism)
• Leishmania infantum (drug effects, metabolism)
• Leishmaniasis, Visceral (drug therapy, metabolism)
• Membrane Potential, Mitochondrial (drug effects)
• Reactive Oxygen Species (metabolism)