Abstract |
The study focuses on the morphological and physiological cell responses to oxidative stress induced by high temperature treatment in the industrially relevant fungus Aspergillus niger 26. Temperatures above 30 °C lead to growth suppression and changes in morphological characteristics: decrease in the size of hyphal elements and increase in "active length" by switching from slightly branched long filaments to a multitude of branched forms containing active cytoplasm. Transmission electron microscopy of fungal cultures heated at 40 °C demonstrated abnormal wavy septation with reduced amount of chitin (as shown by WGA-gold labelling), intrahyphal hyphae development, disintegration of mitochondria and extensive autolysis. Temperature-dependent decrease in the total intracellular protein content and a sharp increase (six to tenfold) in oxidatively damaged proteins were also demonstrated. Elevated temperatures caused a two and threefold increase in catalase and superoxide dismutase activities, respectively.
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Authors | Radoslav Abrashev, Stoyanka Stoitsova, Ekaterina Krumova, Svetlana Pashova, Tsvetelina Paunova-Krasteva, Spassen Vassilev, Pavlina Dolashka-Angelova, Maria Angelova |
Journal | World journal of microbiology & biotechnology
(World J Microbiol Biotechnol)
Vol. 30
Issue 5
Pg. 1661-8
(May 2014)
ISSN: 1573-0972 [Electronic] Germany |
PMID | 24366816
(Publication Type: Journal Article)
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Chemical References |
- Fungal Proteins
- Catalase
- Superoxide Dismutase
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Topics |
- Aspergillus niger
(growth & development, metabolism, ultrastructure)
- Biomass
- Catalase
(metabolism)
- Fungal Proteins
(metabolism)
- Hot Temperature
- Hyphae
(growth & development, ultrastructure)
- Microscopy, Electron, Transmission
- Oxidative Stress
(physiology)
- Superoxide Dismutase
(metabolism)
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