Abstract | BACKGROUND:
Tumor-derived exosomes have been viewed as a source of tumor antigens that can be used to induce anti- tumor immune responses. In the current study, we aim to investigate the regulatory effect of the epigenetic drug MS-275 on hepatoma G2 (HepG2) cell-derived exosomes, especially for their immunostimulatory properties and alteration of some non-specific immune protein expression, such as heat shock protein (HSP) 70, major histocompatibility complex (MHC) class I polypeptide-related sequence A ( MICA) and MICB. METHODS:
MS-275 was used to modulate the secretion of exosomes in human HepG2 cells, and exosomes from untreated HepG2 cells served as negative controls. RT-PCR was used to test the expression of HSP70, MICA and MICB in HepG2 cells. Immunogold labeling of exosomes and western blotting analysis were carried out to compare the expression of HSP70, MICA and MICB proteins in exosomes with or without MS-275 treatment. A natural killer (NK) cell cytotoxicity assay and peripheral blood mononuclear cell (PBMC) proliferation assay were used to evaluate the effect of MS-275 on the immunostimulatory ability of exosomes. RESULTS: Immunogold labeling and western blot analysis showed that modification of MS-275 increased the expression of HSP70 and MICB in exosomes. RT-PCR showed the mRNA levels of HSP70 and MICB were upregulated in HepG2 cells and were consistent with their protein levels in exosomes. The exosomes modified by MS-275 could significantly increase the cytotoxicity of NK cells and proliferation of PBMC (P < 0.05). CONCLUSIONS:
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Authors | Wenhua Xiao, Weiwei Dong, Caihong Zhang, Gaowa Saren, Paili Geng, Huixia Zhao, Quiwen Li, Jianhua Zhu, Guanghui Li, Shufang Zhang, Ming Ye |
Journal | European journal of medical research
(Eur J Med Res)
Vol. 18
Pg. 61
(Dec 23 2013)
ISSN: 2047-783X [Electronic] England |
PMID | 24359553
(Publication Type: Journal Article)
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Chemical References |
- Benzamides
- HSP70 Heat-Shock Proteins
- Histocompatibility Antigens Class I
- MHC class I-related chain A
- MICB antigen
- Pyridines
- entinostat
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Topics |
- Benzamides
(pharmacology, therapeutic use)
- Blotting, Western
- Carcinoma, Hepatocellular
(drug therapy, genetics, immunology, ultrastructure)
- Cell Proliferation
(drug effects)
- Cytotoxicity, Immunologic
(drug effects)
- Epigenesis, Genetic
(drug effects)
- Exosomes
(drug effects, immunology, ultrastructure)
- Gene Expression Regulation, Neoplastic
(drug effects)
- HSP70 Heat-Shock Proteins
(genetics, metabolism)
- Hep G2 Cells
- Histocompatibility Antigens Class I
(genetics, metabolism)
- Humans
- Killer Cells, Natural
(drug effects, immunology)
- Leukocytes, Mononuclear
(drug effects, metabolism, pathology)
- Liver Neoplasms
(drug therapy, genetics, immunology, ultrastructure)
- Pyridines
(pharmacology, therapeutic use)
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