Abstract | OBJECTIVES: In the present study, we aimed to examine whether fractions from an edible sea weed, Hizikia fusiformis, had immunomodulatory effects, particularly an anti-atopic effect, by attenuating the expression of T cell-dependent cytokines using in-vitro and in-vivo animal atopic dermatitis-like models. METHODS: The anti-atopic activities were examined in in vitro, and a 2,4-dinitrochlorobenzene ( DNCB)-induced atopic dermatitis-like mouse model using quantitative real-time polymerase chain reaction, electrophoretic-mobility shift and histopathological analysis. KEY FINDINGS: Our results showed that the final fraction (F2') of H. fusiformis contained a higher amount of butanoic acid which was not found in the other fractions, and effectively inhibited T cell activation by inhibiting dephosphorylation of nuclear factor of activated T cells in electrophoretic-mobility shift assay. As a consequence, helper T cell-dependent cytokines, such as interleukin-2, -4 and interferon-γ, were significantly inhibited while activated with an anti-CD3 antibody. We also showed that skin challenged with DNCB successfully recovered when treated with 2.5 mg/kg, comparable to that by 0.25% prednicarbate. These results indicate that F2' may contribute to inhibit T cell activation by eliminating Th cell-dependent cytokines. CONCLUSIONS: Taken together, we concluded that F2' containing butanoic acid may be a new functional anti-atopic candidate, which probably acts through nuclear factor of activated T cell inactivation mechanisms.
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Authors | Kyu Ho Lee, Hee Jung Kim, Hae Bok Kim, Seung Tae Kim, Young Ri Choi, Da Woom Seo, Jung Min Yu, Su Kil Jang, Sang Moo Kim, Do-Ik Lee, Seong Soo Joo |
Journal | The Journal of pharmacy and pharmacology
(J Pharm Pharmacol)
Vol. 66
Issue 3
Pg. 466-76
(Mar 2014)
ISSN: 2042-7158 [Electronic] England |
PMID | 24354475
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 Royal Pharmaceutical Society. |
Chemical References |
- Antibodies
- CD3 Complex
- Cytokines
- Dinitrochlorobenzene
- Immunologic Factors
- NFATC Transcription Factors
- Plant Extracts
- Butyric Acid
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Topics |
- Animals
- Antibodies
(blood)
- Butyric Acid
(analysis, pharmacology, therapeutic use)
- CD3 Complex
(metabolism)
- Cytokines
(metabolism)
- Dermatitis, Atopic
(chemically induced, drug therapy, immunology, metabolism)
- Dinitrochlorobenzene
- Disease Models, Animal
- Immunologic Factors
(pharmacology, therapeutic use)
- Lymphocyte Activation
(drug effects)
- Male
- Mice
- Mice, Inbred BALB C
- NFATC Transcription Factors
(metabolism)
- Phosphorylation
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Seaweed
(chemistry)
- Skin
(drug effects)
- T-Lymphocytes, Helper-Inducer
(metabolism)
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