The effects of luteolin on the intestinal ischemia/reperfusion injury in mice.

Abstract

The purpose of this study is to investigate the potential protective effect of the flavonoid Luteolin on ischemia-reperfusion (IR) injury in mouse intestine, which has not previously been studied. Twenty-four female C57BL/6 mice were randomly assigned to four groups, each consisting of 6 mice: a sham group (laparotomy, but no IR injury), a sham + Luteolin group (no IR, and Luteolin was administered intraperitoneally 30 min after laparotomy), IR group (30 min occlusion of the superior mesenteric artery (SMA) then 2 hr' reperfusion), IR + Luteolin (30 min occlusion of the SMA then 2 hr' reperfusion; Luteolin was administered intraperitoneally before reperfusion). Intestine tissues were harvested from the mice for histopathological and biochemical analysis. Total oxidant status (TOS) and total antioxidant capacity (TAC) of the intestinal tissues were measured using Erel's method. Oxidative stress index (OSI) was calculated using the TOS/TAC ratio. Intestinal histological changes were significantly decreased in the IR + Luteolin group compared with the IR group (p = .037). TOS tissue levels were also significantly decreased in the IR + Luteolin group compared with the IR group (p = .005). TAC levels did not increase significantly in the IR treatment group and were not affected by Luteolin treatment (p > .05). The results of this study show that Luteolin administration provides considerable protection against IR injury in the mouse intestine.

• Authors: Barış Rafet Karakaş, Fatih Davran, Gülsüm Özlem Elpek, S Halide Akbaş, Kemal Hakan Gülkesen, Nurullah Bülbüller
• Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (J Invest Surg) Vol. 27 Issue 5 Pg. 249-55 (Oct 2014) ISSN: 1521-0553 [Electronic] United States
• PMID: 24354416 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Oxidants
• Luteolin

Topics

• Animals
• Antioxidants (metabolism, pharmacology)
• Female
• Intestines (blood supply, drug effects, injuries)
• Luteolin (pharmacology)
• Mesenteric Artery, Superior (injuries)
• Mice
• Mice, Inbred C57BL
• Oxidants (metabolism)
• Oxidative Stress (drug effects)
• Reperfusion Injury (metabolism, pathology, prevention & control)