Abstract |
In previous studies we showed that a recombinant Modified Vaccinia Ankara (MVA) virus expressing the protein VP2 of AHSV serotype 4 (MVA-VP2) induced virus neutralising antibodies in horses and protected interferon alpha receptor gene knock-out mice (IFNAR-/-) against challenge. We continued these studies and determined, in the IFNAR-/- mouse model, whether the antibody responses induced by MVA-VP2 vaccination play a key role in protection against AHSV. Thus, groups of mice were vaccinated with wild type MVA (MVA-wt) or MVA-VP2 and the antisera from these mice were used in a passive immunisation experiment. Donor antisera from (a) MVA-wt; (b) MVA-VP2 vaccinated; or (c) MVA-VP2 vaccinated and AHSV infected mice, were transferred to AHSV non-immune recipient mice. The recipients were challenged with virulent AHSV together with MVA-VP2 vaccinated and MVA-wt vaccinated control animals and the levels of protection against AHSV-4 were compared between all these groups. The results showed that following AHSV challenge, mice that were passively immunised with MVA-VP2 vaccinated antisera were highly protected against AHSV disease and had lower levels of viraemia than recipients of MVA-wt antisera. Our study indicates that MVA-VP2 vaccination induces a highly protective humoral immune response against AHSV.
|
Authors | Eva Calvo-Pinilla, Francisco de la Poza, Simon Gubbins, Peter Paul Clement Mertens, Javier Ortego, Javier Castillo-Olivares |
Journal | Virus research
(Virus Res)
Vol. 180
Pg. 23-30
(Feb 13 2014)
ISSN: 1872-7492 [Electronic] Netherlands |
PMID | 24333835
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- Capsid Proteins
- Drug Carriers
- VP2 protein, African horse sickness virus
- Viral Vaccines
|
Topics |
- African Horse Sickness
(immunology, prevention & control)
- African Horse Sickness Virus
(genetics, immunology)
- Animals
- Antibodies, Neutralizing
(blood)
- Antibodies, Viral
(blood)
- Capsid Proteins
(genetics, immunology)
- Disease Models, Animal
- Drug Carriers
- Immunization, Passive
- Mice
- Mice, Knockout
- Vaccinia virus
(genetics)
- Viral Vaccines
(administration & dosage, genetics, immunology)
- Viremia
(prevention & control)
|