Expression of claudin-7 and loss of claudin-18 correlate with poor prognosis in gastric cancer.

Abstract

BACKGROUND:

The purpose of this study was to evaluate the expression of claudin-3, claudin-7, and claudin-18 in gastric cancer and to determine the significance of these proteins for patient outcome.

MATERIALS AND METHODS:

A total of 134 samples were obtained from surgically resected specimens from patients who were diagnosed with gastric carcinoma at a single institution. Paraffin tissue sections from tissue microarray blocks were examined with immunohistochemistry for the expression of claudin-3, claudin-7, and claudin-18.

RESULTS:

In normal gastric tissues, positive immunoreactivity was detected for claudin-18 but not for claudin-3 or claudin-7. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of the gastric cancer tissues, respectively. However, 51.5% of gastric cancer tissues exhibited reduced expression of claudin-18. Claudin-7 expression was significantly lower in cases with diffuse histologic type and positive lymphatic invasion. There was a significant inverse correlation between claudin-18 expression and perineural invasion. In the survival analysis, the overall survival time was shorter in patients with claudin-7 expression than in those without claudin-7 expression. However, the overall survival was longer in patients with claudin-18 expression than in those without claudin-18 expression.

CONCLUSIONS:

Our data suggest that the up-regulation of claudin-3 and claudin-7 and the down-regulation of claudin-18 may play a role in the carcinogenesis of gastric cancer. Furthermore, the expression of claudin-7 and the loss of claudin-18 may be independent indicators of a poor prognosis in patients with gastric cancer.

• Authors: Kyong-Hwa Jun, Ji-Hyun Kim, Ji-Han Jung, Hyun-Joo Choi, Hyung-Min Chin
• Journal: International journal of surgery (London, England) (Int J Surg) Vol. 12 Issue 2 Pg. 156-62 ( 2014) ISSN: 1743-9159 [Electronic] England
• PMID: 24333468 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.

Chemical References

• CLDN18 protein, human
• CLDN7 protein, human
• Claudins

Topics

• Aged
• Claudins (biosynthesis)
• Female
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Prognosis
• Stomach Neoplasms (metabolism)
• Tissue Array Analysis