Abstract |
Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is a part of the assembly protein σ2 (AP-2) complex which is involved in clathrin-mediated endocytosis. Quantitative RT-PCR (qRT-PCR) revealed that the clathrin coat AP17 gene was up-regulated 3-fold at 12 h post YHV infection. In addition, immunofluorescence microscopy showed that clathrin coat AP17 was highly expressed in the cytoplasm of the YHV-infected hemocytes. Knockdown of the clathrin coat AP17 gene dramatically reduced YHV replicativity by 32-fold. Interestingly, shrimp pre-treated with chlorpromazine, a commercial drug that inhibits clathrin-dependent endocytosis, exhibited significantly low levels of YHV infection. Taken together, these results suggest that clathrin-mediated endocytosis is involved in YHV propagation in P. monodon.
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Authors | Thapanan Jatuyosporn, Premruethai Supungul, Anchalee Tassanakajon, Kuakarun Krusong |
Journal | Developmental and comparative immunology
(Dev Comp Immunol)
Vol. 44
Issue 1
Pg. 100-10
(May 2014)
ISSN: 1879-0089 [Electronic] United States |
PMID | 24333440
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- Adaptor Protein Complex 2
- Adaptor Proteins, Vesicular Transport
- Clathrin
- RNA, Small Interfering
- Chlorpromazine
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Topics |
- Adaptor Protein Complex 2
(genetics, metabolism)
- Adaptor Proteins, Vesicular Transport
(genetics, metabolism)
- Animals
- Cells, Cultured
- Chlorpromazine
(administration & dosage, pharmacology)
- Clathrin
(metabolism)
- Cytoplasm
(metabolism)
- Endocytosis
(drug effects)
- Hemocytes
(immunology, virology)
- Nidovirales Infections
(immunology)
- Penaeidae
(immunology)
- Protein Transport
- RNA, Small Interfering
(genetics)
- Roniviridae
(physiology)
- Up-Regulation
- Virus Replication
(drug effects, genetics)
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