Human autoantibodies reactive with the signal-recognition particle.

Abstract

We describe autoantibodies reactive with the signal-recognition particle (SRP) in serum of a patient with polymyositis. The serum specifically immunoprecipitated the SRP from human erythroleukemia (K562) cell extracts. Analysis of immunoblots revealed that the serum contained autoantibodies specific for the 54-kDa protein of the SRP but had little or no reactivity with its other five proteins. Indirect immunofluorescence of human laryngeal carcinoma (HEp-2) cells confirmed that the ribonucleoprotein immunoprecipitated by this serum is found mainly in the cytoplasm. This autoimmune serum may be useful for studying the function of the 54-kDa subunit of the SRP in binding to signal sequences or in interacting with other components of the translational machinery.

• Authors: W H Reeves, S K Nigam, G Blobel
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 83 Issue 24 Pg. 9507-11 (Dec 1986) ISSN: 0027-8424 [Print] United States
• PMID: 2432596 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Autoantibodies
• RNA, Small Cytoplasmic
• Ribonucleoproteins
• Signal Recognition Particle
• RNA

Topics

• Antibody Specificity
• Autoantibodies (immunology)
• Autoimmune Diseases (immunology)
• Cytoplasm (immunology)
• Humans
• Myositis (immunology)
• RNA (immunology)
• RNA, Small Cytoplasmic
• Ribonucleoproteins (immunology)
• Signal Recognition Particle