Abstract | PURPOSE: MATERIALS: Cell migration was determined by wound healing assay. Apoptosis was analyzed using annexin V-PE/7-ADD staining as well as Hoechst 33342 staining. Changes of mitochondria membrane potential ( MMP) was evaluated by flow cytometry. Formation of acidic vesicular organelles (AVOs) during autophagy was observed with fluorescence microscope by MDC staining. Immunofluorescence assays were performed to detect the co-localization of LC3 and Lamp2. Western blotting was employed to analyze the activity of the apoptosis related proteins Caspase-3, PARP, Bax and Bcl-2, as well as the autophagy associated processing of LC3-I to LC3-II and Beclin-1 expression. RESULTS: Ce6 mediated SPDT further enhanced cell migration inhibition, significantly triggered cell apoptosis, nuclear condensation and MMP drop. Cleaved Caspase-3 and PARP increased dramatically after Ce6-SPDT, accompanied by decreased Bcl-2 expression, while the expression of Bax remained stable. Additionally, AVOs formation, co-localization of LC3 and Lamp2 occurred following Ce6-SPDT and simultaneously accompanied by LC3-II processing and increased Beclin-1 expression. CONCLUSIONS: Ce6-SPDT could enhance cell migration inhibition, and induce mitochondria-dependent apoptosis as well as autophagy in 4T1 cells.
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Authors | Qing Li, Quanhong Liu, Pan Wang, Xiaolan Feng, Haiping Wang, Xiaobing Wang |
Journal | Ultrasonics
(Ultrasonics)
Vol. 54
Issue 4
Pg. 981-9
(Apr 2014)
ISSN: 1874-9968 [Electronic] Netherlands |
PMID | 24321299
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Chlorophyllides
- Porphyrins
- phytochlorin
|
Topics |
- Animals
- Apoptosis
(drug effects, radiation effects)
- Autophagy
(drug effects, radiation effects)
- Blotting, Western
- Cell Line, Tumor
- Cell Movement
(drug effects, radiation effects)
- Cell Survival
(drug effects, radiation effects)
- Chlorophyllides
- Flow Cytometry
- Immunohistochemistry
- Mammary Neoplasms, Experimental
(drug therapy, radiotherapy)
- Membrane Potentials
- Mice
- Microscopy, Fluorescence
- Photochemotherapy
(methods)
- Porphyrins
(pharmacology)
- Ultrasonic Therapy
(methods)
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