Walker 256/B malignant breast cancer cells disrupt osteoclast cytomorphometry and activity in rats: modulation by α-tocopherol acetate.

Abstract

We examined the effects of vitamin E supplementation (VES) on osteoclast (OC) resorbing activity and cytomorphometry in Walker 256/B tumor osteolytic rats. Twenty-four aged male rats were randomized into 3 groups: 6 were sham operated; 9 were injected in the right hind limb with Walker 256/B cells (W256 group); and 9 were injected as above and supplemented with VE (45mg/kg BW) (W256VE group). Twenty days later, bone mass (BV/TV) and some microarchitectural parameters were assessed. Some histodynamic parameters, cellular and nuclear form factors (FFC and FFN), and nuclear-cytoplasmic ratio (N/C) of OC were measured for each group. W256 group exhibited osteolytic lesions in the operated femora. Walker 256/B induced trabecular perforation and decreased BV/TV associated with significant increases in OC numbering (N.Oc/B.Ar and Oc.N/B.Pm) and activity (ES/BS and Oc.S/BS). While FFN remain unchanged, the FFC and N/C ratio increased in the W256 group. W256VE showed less osteolytic lesions. Moreover, disruption of bone microarchitecture and OC activity in W256VE group decreased. VES reduced the malignant Walker 256/B-induced enhanced OC resorbing activity with cytoinhibition rate reaching 41%. The protective effect of VE may be due to its modulation of OC cytomorphometry and subsequently their activity.

• Authors: Riadh Badraoui, Hmed Ben-Nasr, Selma Amamou, Michèle Véronique El-May, Tarek Rebai
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 210 Issue 3 Pg. 135-41 (Mar 2014) ISSN: 1618-0631 [Electronic] Germany
• PMID: 24314812 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier GmbH. All rights reserved.

Chemical References

• Biomarkers
• Isoenzymes
• Vitamins
• Acid Phosphatase
• Tartrate-Resistant Acid Phosphatase
• alpha-Tocopherol

Topics

• Acid Phosphatase (metabolism)
• Animals
• Biomarkers (metabolism)
• Bone Neoplasms (metabolism, prevention & control, secondary)
• Bone Remodeling (drug effects)
• Breast Neoplasms, Male (complications, drug therapy, pathology)
• Dietary Supplements
• Femur (drug effects, metabolism, pathology)
• Isoenzymes (metabolism)
• Male
• Osteoclasts (drug effects, metabolism, pathology)
• Osteolysis (etiology, metabolism, pathology, prevention & control)
• Rats
• Rats, Sprague-Dawley
• Tartrate-Resistant Acid Phosphatase
• Time Factors
• Vitamins (pharmacology)
• alpha-Tocopherol (pharmacology)