Effect of pheniramine maleate on reperfusion injury in brain tissue.

Abstract

BACKGROUND:

The aim of this study was to investigate the protective effects of methylprednisolone (Pn), which is a potent anti-inflammatory agent, and pheniramine maleate (Ph), which is an antihistaminic with some anti-inflammatory effects, on reperfusion injury in brain developing after ischemia of the left lower extremity of rats.

MATERIAL AND METHODS:

Twenty-eight randomly selected male Sprague-Dawley rats were divided into 4 groups: Group 1 was the control group, Group 2 was the sham group (I/R), Rats in Group 3 were subjected to I/R and given Ph, and rats in Group 4 were subjected to I/R and given Pn. A tourniquet was applied at the level of left groin region of subjects in the I/R group after induction of anesthesia. One h of ischemia was performed with no drug administration. In the Ph group, half of a total dose of 10 mg/kg Ph was administered intraperitoneally before ischemia and the remaining half before reperfusion. In the Pn group, subjects received a single dose of 50 mg/kg Pn intraperitoneally at the 30th min of ischemia. Brains of all subjects were removed after 24 h for examination.

RESULTS:

Malondialdehyde (MDA) levels of the prefrontal cortex were significantly lower in the Ph group than in the I/R group (p<0.05). Superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities were found to be significantly higher in the Ph group than in the I/R group (p<0.05). Histological examination demonstrated that Ph had protective effects against I/R injury developing in the brain tissue.

CONCLUSIONS:

Ph has a protective effect against ischemia/reperfusion injury created experimentally in rat brains.

• Authors: Ismail Yürekli, Orhan Gökalp, Müge Kiray, Gamze Gökalp, Kazım Ergüneş, Ebru Salman, Banu Sarer Yürekli, Ismail Safa Satoğlu, Yüksel Beşir, Habib Cakır, Ali Gürbüz
• Journal: Medical science monitor basic research (Med Sci Monit Basic Res) Vol. 19 Pg. 285-90 (Dec 06 2013) ISSN: 2325-4416 [Electronic] United States
• PMID: 24309384 (Publication Type: Journal Article)

Chemical References

• Histamine H1 Antagonists
• Pheniramine
• Malondialdehyde

Topics

• Animals
• Brain Injuries (drug therapy, metabolism)
• Histamine H1 Antagonists (therapeutic use)
• In Situ Nick-End Labeling
• Male
• Malondialdehyde (metabolism)
• Pheniramine (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy)