Abstract |
In this study, the Actinobacillus pleuropneumoniae antigens ApxIA, ApxIIA, ApxIIIA and OmpA were expressed in an attenuated strain of Salmonella (∆lon∆cpxR∆asd) for prevention of porcine pleuropneumonia. In order to evaluate the immunization strategy of the construct, a total 60 BALB/c mice were equally divided into four groups (n = 15). Group A mice were intranasally immunized only at 6-weeks-of-age, while group B mice were intransally primed and boosted at 6- and 9-weeks-of-age, respectively, and group C mice were intransally primed at 6-weeks-of-age and subsequently boosted twice at 9- and 12-weeks-of-age. Group D mice were used as a control, which were inoculated with sterile PBS. Groups A, B, and C showed significantly higher serum IgG and fecal IgA immune responses than those of the control group. After virulent challenge with a wild type A. pleuropneumoniae, the immunized groups A, B and C showed 33.3 %, 13.3 % and 26.7 % mortality as the control group showed 60 % mortality. These results showed that the protection against porcine pleuropneumonia using the construct can be optimized by a double intranasal vaccination.
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Authors | Jin Hur, John Hwa Lee |
Journal | Veterinary research communications
(Vet Res Commun)
Vol. 38
Issue 1
Pg. 87-91
(Mar 2014)
ISSN: 1573-7446 [Electronic] Netherlands |
PMID | 24307459
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Bacterial
- Bacterial Vaccines
- Immunoglobulin A
- Immunoglobulin G
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Topics |
- Actinobacillus pleuropneumoniae
(genetics, immunology)
- Administration, Intranasal
- Animals
- Antigens, Bacterial
(administration & dosage, immunology)
- Bacterial Vaccines
(immunology)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Feces
(chemistry)
- Immunity, Humoral
- Immunization
(veterinary)
- Immunoglobulin A
(analysis)
- Immunoglobulin G
(blood)
- Mice
- Mice, Inbred BALB C
- Pleuropneumonia
(mortality, prevention & control, veterinary)
- Salmonella
(genetics)
- Swine
- Swine Diseases
(prevention & control)
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